Macondo crude oil from the Deepwater Horizon oil spill disrupts specific developmental processes during zebrafish embryogenesis.
Macondo crude oil from the Deepwater Horizon oil spill disrupts specific developmental processes during zebrafish embryogenesis.
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DOI：
10.1186/1741-7007-10-40
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发表时间：
2012-05-04
期刊：
影响因子：
5.4
通讯作者：
Barresi MJ
中科院分区：
文献类型：
--
作者：
de Soysa TY;Ulrich A;Friedrich T;Pite D;Compton SL;Ok D;Bernardos RL;Downes GB;Hsieh S;Stein R;Lagdameo MC;Halvorsen K;Kesich LR;Barresi MJ
深水地平线灾难是历史上最大的海洋石油泄漏事件，石油完全垂直暴露于水体表明它可能会影响生态系统的巨大多样性。最脆弱的生物体是那些在生命早期阶段遇到这些污染物的生物体。原油的水溶性成分和特定的多环芳烃已被证明会导致多种硬骨鱼物种的心血管和颅面发育缺陷，但这些缺陷的发育起源尚未确定。我们采用斑马鱼（Danio rerio）作为模型来测试深水地平线石油的积水部分（WAF）是否会影响特定的胚胎发育过程。虽然斑马鱼不是海湾水域的本地物种，但其发育生物学已得到很好的表征，并使其成为揭示马孔多原油毒性背后的细胞和分子机制的强大模型系统。在漏油事件期间采集的马孔多原油样本的 WAF 用于治疗斑马鱼的整个胚胎和幼虫发育过程。我们的研究结果表明，马孔多原油导致斑马鱼胚胎发生中出现多种显着缺陷，但这些缺陷具有特定的发育起源。 WAF 治疗导致颅面发育和循环功能缺陷，与之前的报告类似，但我们扩展了这些结果，表明它们可能源自神经嵴细胞发育的早期缺陷。此外，我们证明暴露于 WAF 会导致特定发育过程中的各种新的变形，包括程序性细胞死亡、运动行为、感觉和运动轴突寻路、体节发生和肌肉模式。有趣的是，经过几个月的重复分析，细胞死亡和肌肉表型的严重程度有所下降，这与油中芳香烃和烷烃成分的快速下降有关。这些致畸效应是否是深水地平线漏油事件所特有的，还是普遍存在于大多数原油类型中的，仍有待确定。这项工作为进一步研究原油介导的变形背后的分子机制建立了一个模型。此外，由于斑马鱼和其他脊椎动物之间遗传和细胞过程的高度保守，我们的工作还提供了一个平台，可以更集中地评估深水地平线漏油事件对墨西哥湾和大西洋本地鱼类早期生命阶段的影响。
The Deepwater Horizon disaster was the largest marine oil spill in history, and total vertical exposure of oil to the water column suggests it could impact an enormous diversity of ecosystems. The most vulnerable organisms are those encountering these pollutants during their early life stages. Water-soluble components of crude oil and specific polycyclic aromatic hydrocarbons have been shown to cause defects in cardiovascular and craniofacial development in a variety of teleost species, but the developmental origins of these defects have yet to be determined. We have adopted zebrafish, Danio rerio, as a model to test whether water accumulated fractions (WAF) of the Deepwater Horizon oil could impact specific embryonic developmental processes. While not a native species to the Gulf waters, the developmental biology of zebrafish has been well characterized and makes it a powerful model system to reveal the cellular and molecular mechanisms behind Macondo crude toxicity. WAF of Macondo crude oil sampled during the oil spill was used to treat zebrafish throughout embryonic and larval development. Our results indicate that the Macondo crude oil causes a variety of significant defects in zebrafish embryogenesis, but these defects have specific developmental origins. WAF treatments caused defects in craniofacial development and circulatory function similar to previous reports, but we extend these results to show they are likely derived from an earlier defect in neural crest cell development. Moreover, we demonstrate that exposure to WAFs causes a variety of novel deformations in specific developmental processes, including programmed cell death, locomotor behavior, sensory and motor axon pathfinding, somitogenesis and muscle patterning. Interestingly, the severity of cell death and muscle phenotypes decreased over several months of repeated analysis, which was correlated with a rapid drop-off in the aromatic and alkane hydrocarbon components of the oil. Whether these teratogenic effects are unique to the oil from the Deepwater Horizon oil spill or generalizable for most crude oil types remains to be determined. This work establishes a model for further investigation into the molecular mechanisms behind crude oil mediated deformations. In addition, due to the high conservation of genetic and cellular processes between zebrafish and other vertebrates, our work also provides a platform for more focused assessment of the impact that the Deepwater Horizon oil spill has had on the early life stages of native fish species in the Gulf of Mexico and the Atlantic Ocean.
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影响因子：
5.5
作者：
CLARKE, JDW;HAYES, BP;ROBERTS, A
通讯作者：
ROBERTS, A
影响因子：
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Berger, Joachim;Berger, Silke;Currie, Peter D.
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Asharani, P. V.;Yi Lianwu;Valiyaveettil, Suresh
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Valiyaveettil, Suresh
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作者：
Alexandre, Paula;Reugels, Alexander M.;Clarke, Jonathan D. W.
通讯作者：
Clarke, Jonathan D. W.
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作者：
BERNHARDT, RR;PATEL, CK;KUWADA, JY
通讯作者：
KUWADA, JY