Regulation of sex steroid receptor gene expression by progesterone and testosterone in cultured human endometrial stromal cells.
Regulation of sex steroid receptor gene expression by progesterone and testosterone in cultured human endometrial stromal cells.
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培养的人子宫内膜基质细胞中孕酮和睾酮对性类固醇受体基因表达的调节。
DOI：
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发表时间：
1995
影响因子：
5.8
通讯作者：
Jun Fujita
中科院分区：
文献类型：
--
作者：
M. Iwai;H. Kanzaki;M. Fujimoto;Katsutoshi Kojima;H. Hatayama;Takuya Inoue;T. Higuchi;Hiroki Nakayama;Takahide Mori;Jun Fujita
孕酮(P)是已知的调节性类固醇受体的子宫细胞。然而，它在信使核糖核酸(MRNA)水平上的精确调控尚不清楚。本研究采用定量逆转录聚合酶链式反应的方法，研究了P和睾酮(T)对培养的人子宫内膜间质细胞(ESC)性激素受体的调节作用。我们从人子宫内膜组织中分离出胚胎干细胞，分别在P(10(-6)m ol/L)或T(10(-8)m o l/L)的作用下培养9天。P可使培养的人胚胎干细胞孕激素受体(PR)、雌激素受体和雄激素受体的mRNA水平分别降低到0.56+/-0.04-、0.53+/-0.08-和0.84+/-0.04倍(平均值+/-SE)。T还可使培养的人胚胎干细胞PR、雌激素受体和雄激素受体的mRNA水平分别降低至0.48+/-0.06-、0.52+/-0.05-和0.82+/-0.04倍(平均值+/-SE)。这些P和T的降低以剂量依赖的方式发生。我们还检测了培养0、3、6和9天的人胚胎干细胞中性类固醇受体的水平。未加P的ESC中PR基因表达水平呈时间依赖性增加。这种增加也被P抑制，在整个培养过程中，P的存在几乎保持不变的mRNA水平。我们的结果表明，P或T是ESC中性类固醇受体的调节因子，这种调节可能影响ESC中蜕膜改变对P的反应性。
Progesterone (P) is known to regulate sex steroid receptors in uterine cells. However, its precise regulation at the messenger ribonucleic acid (mRNA) level is unclear. In this study we examined the effects of P and testosterone (T) on the regulation of sex steroid receptors in cultured human endometrial stromal cells (ESC), using the quantitative reverse transcriptase polymerase chain reaction method. We isolated ESC from human endometrial tissues and cultured them with or without P (10(-6) mol/L) or T (10(-8) mol/L) for 9 days. Incubation with P decreased progesterone receptor (PR), estrogen receptor, and androgen receptor mRNA levels in cultured human ESC to 0.56 +/- 0.04-, 0.53 +/- 0.08-, and 0.84 +/- 0.04-fold (mean +/- SE), respectively. T also decreased PR, estrogen receptor, and androgen receptor mRNA levels in cultured human ESC to 0.48 +/- 0.06-, 0.52 +/- 0.05-, and 0.82 +/- 0.04-fold (mean +/- SE), respectively. These decreases by P and T occurred in a dose-dependent manner. We also examined the sex steroid receptor levels in human ESC cultured for 0, 3, 6, and 9 days. The PR mRNA level in ESC without P was increased in a time-dependent manner. This increase was also inhibited by P, and the mRNA level in the presence of P was almost constant throughout the culture period. Our results demonstrated that P or T is a regulator of sex steroid receptors in ESC and that this regulation may influence the responsiveness to P of decidual change in ESC.
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DOI：
10.1097/00006254-198510000-00017
发表时间：
1984-11
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
M. Press;N. Nousek-Goebl;W. King;A. Herbst;G. Greene
通讯作者：
M. Press;N. Nousek-Goebl;W. King;A. Herbst;G. Greene
影响因子：
--
作者：
QUARMBY, VE;YARBROUGH, WG;WILSON, EM
通讯作者：
WILSON, EM
影响因子：
5.8
作者：
LESSEY, BA;KILLAM, AP;MCCARTY, KS
通讯作者：
MCCARTY, KS
影响因子：
6.1
作者：
HORIE, K;TAKAKURA, K;MORI, T
通讯作者：
MORI, T
影响因子：
56.9
作者：
GREENE, GL;GILNA, P;SHINE, J
通讯作者：
SHINE, J