Diversity of the Bacterial and Viral Communities in the Tropical Horse Tick, Dermacentor nitens, in Colombia.
Diversity of the Bacterial and Viral Communities in the Tropical Horse Tick, Dermacentor nitens, in Colombia.
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DOI：
10.3390/pathogens12070942
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发表时间：
2023-07-16
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影响因子：
--
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扁虱是必须的嗜血性体外寄生虫，在包括人类在内的各种脊椎动物之间传播病原体。扁虱的微生物和病毒群落，包括致病微生物，众所周知是高度多样化的。然而，推动这种多样性的因素并没有得到很好的理解。热带马虱分布在美洲各地，被认为是引起马的梨形体病的巴贝斯虫和马泰勒氏菌的天然媒介。在这项研究中，我们通过对来自哥伦比亚三个不同地理区域(玻利瓦尔、安蒂奥基亚和科尔多瓦)的马匹的被动调查，表征了与部分喂养的尼氏革螨雌性动物相关的细菌和病毒群落。使用Illumina-MiSeq平台(美国加利福尼亚州圣地亚哥Illumina)进行16S rRNA基因V3和V4高变区的RNA-seq和测序。共鉴定出356个可操作分类单元(OTU)，其中主要发现内生共生菌--粉菌科(Francisellaceae/Francisella spp.)在丘状病毒科、横纹病毒科和黄病毒科三个病毒科中鉴定了对应于六种不同病毒的九个重叠群。发现不同地理区域之间微生物组成的相对丰度的差异与是否存在类弗朗西塞氏菌内共生菌(FLE)无关。在每个地区发现的最常见的细菌是玻利瓦尔的棒状杆菌、安蒂奥基亚的葡萄球菌和科尔多瓦的假单胞菌。在科尔多瓦的样本中发现了类立克次体内共生菌，主要被认为是哥伦比亚立克次体病的病原体。后转录组学发现了13个含有fle基因的重叠群，这表明了地区差异的趋势。这些发现表明，扁虱及其细菌组成之间存在地区差异。
Ticks are obligatory hematophagous ectoparasites that transmit pathogens among various vertebrates, including humans. The microbial and viral communities of ticks, including pathogenic microorganisms, are known to be highly diverse. However, the factors driving this diversity are not well understood. The tropical horse tick, Dermacentor nitens, is distributed throughout the Americas and it is recognized as a natural vector of Babesia caballi and Theileria equi, the causal agents of equine piroplasmosis. In this study, we characterized the bacterial and viral communities associated with partially fed Dermacentor nitens females collected using a passive survey on horses from field sites representing three distinct geographical areas in the country of Colombia (Bolivar, Antioquia, and Cordoba). RNA-seq and sequencing of the V3 and V4 hypervariable regions of the 16S rRNA gene were performed using the Illumina-Miseq platform (Illumina, San Diego, CA, USA). A total of 356 operational taxonomic units (OTUs) were identified, in which the presumed endosymbiont, Francisellaceae/Francisella spp., was predominantly found. Nine contigs corresponding to six different viruses were identified in three viral families: Chuviridae, Rhabdoviridae, and Flaviviridae. Differences in the relative abundance of the microbial composition among the geographical regions were found to be independent of the presence of Francisella-like endosymbiont (FLE). The most prevalent bacteria found in each region were Corynebacterium in Bolivar, Staphylococcus in Antioquia, and Pseudomonas in Cordoba. Rickettsia-like endosymbionts, mainly recognized as the etiological agent of rickettsioses in Colombia, were detected in the Cordoba samples. Metatranscriptomics revealed 13 contigs containing FLE genes, suggesting a trend of regional differences. These findings suggest regional distinctions among the ticks and their bacterial compositions.
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DOI：
10.1590/s1517-83822013000300035
发表时间：
2013
期刊：
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
影响因子：
--
作者：
Gonçalves DD;Carreira T;Nunes M;Benitez A;Lopes-Mori FM;Vidotto O;de Freitas JC;Vieira ML
通讯作者：
Vieira ML
影响因子：
3.3
作者：
Gerhart, Jonathan G.;Dutcher, H. Auguste;Raghavan, Rahul
通讯作者：
Raghavan, Rahul
影响因子：
5.7
作者：
Bonnet SI;Binetruy F;Hernández-Jarguín AM;Duron O
通讯作者：
Duron O
影响因子：
3.2
作者：
Clow, Katie M.;Weese, J. Scott;Jardine, Claire M.
通讯作者：
Jardine, Claire M.
影响因子：
4.9
作者：
Duron, Olivier;Binetruy, Florian;Chevillon, Christine
通讯作者：
Chevillon, Christine