Naftifine enhances photodynamic therapy against Staphylococcus aureus by inhibiting staphyloxanthin expression
Naftifine enhances photodynamic therapy against Staphylococcus aureus by inhibiting staphyloxanthin expression
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萘替芬通过抑制葡萄球菌黄素表达增强对金黄色葡萄球菌的光动力治疗
DOI：
10.1016/j.dyepig.2020.108392
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发表时间：
2020-08
影响因子：
4.5
通讯作者：
Zhuo Chen
中科院分区：
文献类型：
--
作者：
Jing Sun;Yuxiang Zhang;Jianqiang Su;Tao Dai;Jincan Chen;Lei Zhang;Huanhuan Wang;Wenzhen Liu;Mingdong Huang;Zhuo Chen
金黄色葡萄球菌（Staphyloxanthin，STX）是由金黄色葡萄球菌（Staphylococcus aureus，S.金黄色葡萄球菌），包括耐甲氧西林S.金黄色葡萄球菌（MRSA）是一种广泛分布的革兰氏阳性细菌，与几种临床人类感染有关。萘替芬是一种广谱抗真菌药物，在光动力抗菌治疗（PACT）中起重要作用。金黄色。STX可减弱PACT对S.金黄色葡萄球菌素的活性与其清除活性氧（ROS）的生物学功能密切相关。S.与S. aureusto PACT相比，用萘替芬（10 μM）孵育后，aureusto PACT显著提高。金黄色未经萘替芬治疗。这种现象的原因是萘替芬抑制STX生物合成并改善膜通透性。综上所述，我们确定STX是金黄色葡萄球菌PACT的限制因素。同时，我们发现PACT与STX生物合成抑制剂萘替芬联合使用对S具有协同抗菌作用。为快速、有效地治疗金黄色葡萄球菌提供了新的途径。金黄色葡萄球菌感染无耐药性。
Staphyloxanthin (STX) is a virulence factor produced byStaphylococcus aureus(S. aureus) including methicillin-resistantS. aureus(MRSA), a widely distributed Gram-positive bacterium related to several clinical human infections. We herein reported that Naftifine, a broad-spectrum antifungal agent, plays an important role on photodynamic antimicrobial therapy (PACT) forS. aureus. STX attenuated the PACT effect onS. aureusin a dose-dependent manner, which probably due to its biological function of scavenging reactive oxygen species (ROS). On the contrary, the sensitivity ofS. aureusto PACT was significantly improved after an incubation with Naftifine (10 μM) compared with that ofS. aureusuntreated with Naftifine. The reason for this phenomenon is that Naftifine inhibits STX biosynthesis and improves membrane permeability. Taken together, we identified STX as a limiting factor of PACT inS. aureus. Meanwhile, we found that the combination of PACT and Naftifine, an inhibitor of STX biosynthesis, has synergistic antibacterial effect onS. aureus, and provide a new way for the rapid and efficient treatment ofS. aureusinfection without drug resistance.
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影响因子：
3.3
作者：
Kossakowska, Monika;Nakonieczna, Joanna;Grinholc, Mariusz
通讯作者：
Grinholc, Mariusz
影响因子：
5
作者：
Yaxin Zhang;Ke Zheng;Zhuo Chen;Jincan Chen;Ping Hu;Linrong Cai;Zafar Iqbal;Mingdong Huang
通讯作者：
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影响因子：
5.4
作者：
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通讯作者：
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影响因子：
3.9
作者：
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通讯作者：
Lijun Xue;Y. Chen;Zhiyun Yan;W. Lu;Dong Wan;Huifeng Zhu
影响因子：
3.9
作者：
Coker, Melanie S.;Forbes, Louisa V.;Kettle, Anthony J.
通讯作者：
Kettle, Anthony J.