Complex carbohydrate utilization by the healthy human microbiome.
Complex carbohydrate utilization by the healthy human microbiome.
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DOI：
10.1371/journal.pone.0028742
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Henrissat B
中科院分区：
文献类型：
--
作者：
Cantarel BL;Lombard V;Henrissat B
人体内不同的生态环境为微生物提供了广泛的营养来源和生存挑战。DNA测序等技术的进步，使我们能够比过去更深入地了解人类微生物群的分子功能。在这里，我们旨在研究人类微生物组中切割复合碳水化合物的酶（CAZymes），以确定(i)细菌基因组的CAZyme谱是否在身体部位或细菌家族中更相似，以及（ii）生活在不同人类栖息地的微生物群落的糖降解和利用能力。通过对来自12个人类栖息地的493个细菌参考基因组的研究，我们发现这些细菌的糖降解能力与同一细菌科的其他细菌相比更相似。然而，对来自5个主要身体部位的520个宏基因组样本的分析表明，即使群落组成不同，CAZyme谱在一个身体部位也非常相似，这表明观察到的功能谱和微生物栖息地已经适应了当地的碳水化合物组成。当比较五个主要身体部位对糖的广泛利用时，胃肠道对总糖的降解潜力最大，而葡聚糖和肽聚糖的降解分别在口腔和阴道部位最高。我们的分析表明，每个身体部位的碳水化合物组成具有深远的影响，并且可能构成了形成群落组成的主要驱动力之一，从而形成了当地微生物群落的CAZyme谱，这反过来反映了微生物组对身体部位的适应性。
The various ecological habitats in the human body provide microbes a wide array of nutrient sources and survival challenges. Advances in technology such as DNA sequencing have allowed a deeper perspective into the molecular function of the human microbiota than has been achievable in the past. Here we aimed to examine the enzymes that cleave complex carbohydrates (CAZymes) in the human microbiome in order to determine (i) whether the CAZyme profiles of bacterial genomes are more similar within body sites or bacterial families and (ii) the sugar degradation and utilization capabilities of microbial communities inhabiting various human habitats. Upon examination of 493 bacterial references genomes from 12 human habitats, we found that sugar degradation capabilities of taxa are more similar to others in the same bacterial family than to those inhabiting the same habitat. Yet, the analysis of 520 metagenomic samples from five major body sites show that even when the community composition varies the CAZyme profiles are very similar within a body site, suggesting that the observed functional profile and microbial habitation have adapted to the local carbohydrate composition. When broad sugar utilization was compared within the five major body sites, the gastrointestinal track contained the highest potential for total sugar degradation, while dextran and peptidoglycan degradation were highest in oral and vaginal sites respectively. Our analysis suggests that the carbohydrate composition of each body site has a profound influence and probably constitutes one of the major driving forces that shapes the community composition and therefore the CAZyme profile of the local microbial communities, which in turn reflects the microbiome fitness to a body site.
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DOI：
10.1126/science.1183605
发表时间：
2010-05-21
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Human Microbiome Jumpstart Reference Strains Consortium;Nelson KE;Weinstock GM;Highlander SK;Worley KC;Creasy HH;Wortman JR;Rusch DB;Mitreva M;Sodergren E;Chinwalla AT;Feldgarden M;Gevers D;Haas BJ;Madupu R;Ward DV;Birren BW;Gibbs RA;Methe B;Petrosino JF;Strausberg RL;Sutton GG;White OR;Wilson RK;Durkin S;Giglio MG;Gujja S;Howarth C;Kodira CD;Kyrpides N;Mehta T;Muzny DM;Pearson M;Pepin K;Pati A;Qin X;Yandava C;Zeng Q;Zhang L;Berlin AM;Chen L;Hepburn TA;Johnson J;McCorrison J;Miller J;Minx P;Nusbaum C;Russ C;Sykes SM;Tomlinson CM;Young S;Warren WC;Badger J;Crabtree J;Markowitz VM;Orvis J;Cree A;Ferriera S;Fulton LL;Fulton RS;Gillis M;Hemphill LD;Joshi V;Kovar C;Torralba M;Wetterstrand KA;Abouellleil A;Wollam AM;Buhay CJ;Ding Y;Dugan S;FitzGerald MG;Holder M;Hostetler J;Clifton SW;Allen-Vercoe E;Earl AM;Farmer CN;Liolios K;Surette MG;Xu Q;Pohl C;Wilczek-Boney K;Zhu D
通讯作者：
Zhu D
DOI：
10.1073/pnas.0806191105
发表时间：
2009-02-10
影响因子：
11.1
作者：
Brulc, Jennifer M.;Antonopoulos, Dionysios A.;White, Bryan A.
通讯作者：
White, Bryan A.
DOI：
10.1038/nbt0885-722
发表时间：
1985-01-01
期刊：
BIO-TECHNOLOGY
影响因子：
--
作者：
HENRISSAT, B;DRIGUEZ, H;SCHULEIN, M
通讯作者：
SCHULEIN, M
影响因子：
6.8
作者：
Henrissat, B;Davies, G
通讯作者：
Davies, G
影响因子：
4.3
作者：
LAINE, RA
通讯作者：
LAINE, RA