De novo sequencing of tree peony (Paeonia suffruticosa) transcriptome to identify critical genes involved in flowering and floral organ development
De novo sequencing of tree peony (Paeonia suffruticosa) transcriptome to identify critical genes involved in flowering and floral organ development
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对牡丹（Paeonia suffruticosa）转录组进行从头测序，以鉴定参与开花和花器官发育的关键基因
DOI：
10.1186/s12864-019-5857-0
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发表时间：
2019-07
期刊：
影响因子：
4.4
通讯作者：
Zhang Xiuxin
中科院分区：
文献类型：
--
作者：
Wang Shunli;Gao Jie;Xue Jingqi;Xue Yuqian;Li D;an;Guan Yanren;Zhang Xiuxin
牡丹是世界著名的观赏花卉，花大而色艳，花型丰富。然而，牡丹花期较短且较整齐，严重影响了牡丹的应用和生产。然而，调控牡丹开花时间和花器官发育的分子机制尚未阐明。由于基因组信息的情况下，454为基础的转录组序列技术从头转录组学被用来确定关键开花基因使用再开花，非再开花，和野生品种的peonies.ResultsA共29,275 unigenes从芽转录组，与776 bp的N50。单基因平均长度为677.18 bp，最长为5815 bp。NCBI-NR、Swiss-Prot、COG、GO和KEGG分别对22，823、17，321、13，312、20，041和9940个单基因进行了功能注释。通过生物信息学、RT-PCR和RACE等方法对其中的64个开花相关基因进行了分析。通过比较任意两个样品的DEG，以及不同发育阶段的芽、分化芽和GA或春化处理的芽中重要成花基因的表达水平，提出了可能的成花诱导途径的遗传网络和花器官发育模型。牡丹开花受长日照、春化、自主、年龄和赤霉素等5条途径的调控，花器官发育遵循ABCE模型。此外，还发现基因PsAP 1，PsCOL 1，PsF 1，PsF 2，PsFT，PsLFY，PsLHY，PsGI，PsSOC 1，PsVIN 3可能调控牡丹的再开花。结论本研究首次提供了牡丹开花相关基因的全面报告，并调查了关键开花相关基因在不同品种的芽，发育阶段，分化的原基，和花部分的表达水平。这些结果为深入研究花器官发育和花期调控的分子机制提供了重要的理论依据。
BackgroundTree peony (Paeonia suffruticosaAndrews) is a globally famous ornamental flower, with large and colorful flowers and abundant flower types. However, a relatively short and uniform flowering period hinders the applications and production of ornamental tree peony. Unfortunately, the molecular mechanism of regulating flowering time and floral organ development in tree peony has yet to be elucidated. Because of the absence of genomic information, 454-based transcriptome sequence technology for de novo transcriptomics was used to identify the critical flowering genes using re-blooming, non-re-blooming, and wild species of tree peonies.ResultsA total of 29,275 unigenes were obtained from the bud transcriptome, with an N50 of 776 bp. The average length of unigenes was 677.18 bp, and the longest sequence was 5815 bp. Functional annotation showed that 22,823, 17,321, 13,312, 20,041, and 9940 unigenes were annotated by NCBI-NR, Swiss-Prot, COG, GO, and KEGG, respectively. Within the differentially expressed genes (DEGs) 64 flowering-related genes were identified and some important flowering genes were also characterized by bioinformatics methods, reverse transcript polymerase chain reaction (RT-PCR), and rapid-amplification of cDNA ends (RACE). Then, the putative genetic network of flowering induction pathways and a floral organ development model were put forward, according to the comparisons of DEGs in any two samples and expression levels of the important flowering genes in differentiated buds, buds from different developmental stages, and with GA or vernalization treated. In tree peony, five pathways (long day, vernalization, autonomous, age, and gibberellin) regulated flowering, and the floral organ development followed an ABCE model. Moreover, it was also found that the genesPsAP1,PsCOL1,PsCRY1,PsCRY2,PsFT,PsLFY,PsLHY,PsGI,PsSOC1, andPsVIN3probably regulated re-blooming of tree peony.ConclusionThis study provides a comprehensive report on the flowering-related genes in tree peony for the first time and investigated the expression levels of the critical flowering related genes in buds of different cultivars, developmental stages, differentiated primordium, and flower parts. These results could provide valuable insights into the molecular mechanisms of flowering time regulation and floral organ development.
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影响因子：
5.3
作者：
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影响因子：
2.6
作者：
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通讯作者：
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影响因子：
2.7
作者：
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通讯作者：
Chen FC
影响因子：
1.2
作者：
P. M. P. Mornya-P.-M.-P.-Mornya-87649655;F. Cheng
通讯作者：
P. M. P. Mornya-P.-M.-P.-Mornya-87649655;F. Cheng
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影响因子：
6.2
作者：
Zhang, Yuan;Zhang, Shougong;Qi, Liwang
通讯作者：
Qi, Liwang