Flow-through microfluidic immunosensors with refractive index-matched silica monoliths as volumetric optical detection elements.
Flow-through microfluidic immunosensors with refractive index-matched silica monoliths as volumetric optical detection elements.
复制标题
DOI：
10.1016/j.snb.2017.07.137
复制
发表时间：
2018-01
期刊：
影响因子：
--
通讯作者：
DeVoe DL
中科院分区：
文献类型：
--
作者：
Wiederoder MS;Kendall EL;Han JH;Ulrich RG;DeVoe DL
本研究利用非原位功能化多孔二氧化硅整体作为体积光学检测元件，建立了一种灵敏、快速的基于吸收的免疫传感器。多孔整料结构有利于高捕获探针密度和短扩散长度尺度，使得能够进行灵敏和快速的测定。在集成到密封的热塑性微流体装置中之前，用芯片外的免疫捕获探针合成和功能化的二氧化硅整料用于在通过多孔结构的灌注期间捕获靶抗原。将金纳米颗粒免疫缀合物与银增强剂组合以产生微米级银簇，然后灌注蔗糖水溶液以限制光散射并增强光信号。使用这种方法，夹心测定法的检测限低至1 ng/mL，动态范围至少为4 log。结果证实，芯片上折射率匹配与功能化多孔二氧化硅整料的组合可以实现简单实用的流通免疫测定，用于灵敏和快速检测靶抗原。
A sensitive and rapid absorbance based immunosensor that utilizes ex situ functionalized porous silica monoliths as volumetric optical detection elements is demonstrated in this study. The porous monolith structure facilitates high capture probe density and short diffusion length scales, enabling sensitive and rapid assays. Silica monoliths, synthesized and functionalized with immunocapture probes off-chip before integration into a sealed thermoplastic microfluidic device, serve to capture target antigens during perfusion through the porous structure. Gold nanoparticle immunoconjugates are combined with silver enhancement to create microscale silver clusters, followed by perfusion of an aqueous sucrose solution to limit light scattering and enhance optical signal. Using this approach, detection limits as low as 1 ng/mL are achieved for a sandwich assay, with a dynamic range of at least 4 logs. The results confirm that the combination of on-chip index matching with functionalized porous silica monoliths can enables simple and practical flow-through immunoassays for the sensitive and rapid detection of target antigens.
登录
查看更多内容
影响因子：
2.9
作者：
Rucker, VC;Havenstrite, KL;Herr, AE
通讯作者：
Herr, AE
影响因子：
3.8
作者：
Sharma, Himanshu;Diep Nguyen;Chen, Aaron;Lew, Valerie;Khine, Michelle
通讯作者：
Khine, Michelle
影响因子：
9.5
作者：
Geissler, Matthias;Roy, Emmanuel;Veres, Teodor
通讯作者：
Veres, Teodor
影响因子：
2.2
作者：
Liang, RQ;Tan, CY;Ruan, KC
通讯作者：
Ruan, KC
影响因子：
6.1
作者：
Chen, C. F.;Liu, J.;DeVoe, D. L.
通讯作者：
DeVoe, D. L.