Rice ragged stunt virus-induced apoptosis affects virus transmission from its insect vector, the brown planthopper to the rice plant.
Rice ragged stunt virus-induced apoptosis affects virus transmission from its insect vector, the brown planthopper to the rice plant.
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水稻褴褛矮化病毒诱导的细胞凋亡影响病毒从其昆虫载体褐飞虱向水稻植株的传播
DOI：
10.1038/srep11413
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发表时间：
2015-06-15
影响因子：
4.6
通讯作者：
Zhang CX
中科院分区：
文献类型：
--
作者：
Huang HJ;Bao YY;Lao SH;Huang XH;Ye YZ;Wu JX;Xu HJ;Zhou XP;Zhang CX
大多数严重损害农作物的植物病毒是由昆虫传播的。然而，使病毒通过昆虫媒介传播的机制知之甚少。褐飞虱（Nilaparvata lugens）是水稻上最严重的害虫之一，它通过吸食水稻韧皮部汁液和传播水稻裂叶矮缩病毒（Rice ragged stunt virus，RRSV）等病毒，对水稻造成严重危害。本研究利用末端脱氧核苷酸转移酶dUTP缺口末端标记技术和RNA干扰技术，研究了RRSV从昆虫载体到水稻体内的传播机制。RRSV可诱导其昆虫载体N. lugens。RSV诱导的细胞凋亡是通过caspase依赖的方式调节的，抑制N。lugens caspase-1基因显著干扰病毒传播。我们的研究结果建立了病毒相关的细胞凋亡和病毒从昆虫载体到宿主植物的传播之间的联系。
Most plant viruses that seriously damage agricultural crops are transmitted by insects. However, the mechanisms enabling virus transmission by insect vectors are poorly understood. The brown planthopper (Nilaparvata lugens) is one of the most serious rice pests, causing extensive damage to rice plants by sucking the phloem sap and transmitting viruses, including Rice ragged stunt virus (RRSV). In this study, we investigated the mechanisms of RRSV transmission from its insect vector to the rice plant in vivo using the terminal deoxynucleotidyl transferase dUTP nick-end labeling assay and RNA interference technology. RRSV induced apoptosis in the salivary gland cells of its insect vector, N. lugens. The RRSV-induced apoptosis was regulated through a caspase-dependent manner, and inhibition of the expression of N. lugens caspase-1 genes significantly interfered with virus transmission. Our findings establish a link between virus-associated apoptosis and virus transmission from the insect vector to the host plant.
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