Polycaprolactone coated porous tricalcium phosphate scaffolds for controlled release of protein for tissue engineering.
Polycaprolactone coated porous tricalcium phosphate scaffolds for controlled release of protein for tissue engineering.
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DOI：
10.1002/jbm.b.31464
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发表时间：
2009-11
影响因子：
3.4
通讯作者：
Bose, Susmita
中科院分区：
文献类型：
--
作者：
Xue, Weichang;Bandyopadhyay, Amit;Bose, Susmita
将聚己内酯（PCL）包被在多孔磷酸三钙（TCP）支架上以实现蛋白质的受控递送。采用网状聚氨酯泡沫作为牺牲支架，制备了孔隙率为70 ~ 90vol %的多孔TCP支架。采用浸渍干燥法将PCL包覆在烧结多孔TCP支架上。PCL涂层后TCP支架抗压强度显著提高。包覆5% PCL溶液的TCP支架在孔隙率为70%时强度最高，为2.41 MPa。模型蛋白牛血清白蛋白（BSA）被有效地包裹在PCL涂层内。BSA包封量由PCL包衣中不同蛋白质组成控制。FTIR分析证实，在PCL涂层过程中，BSA保持了其结构构象，没有表现出明显的变性。在磷酸盐缓冲液中的释放动力学表明，蛋白质的释放是可控的和持续的，主要取决于包裹在PCL涂层中的蛋白质浓度。
Polycaprolactone (PCL) was coated on porous tricalcium phosphate (TCP) scaffolds to achieve controlled protein delivery. Porous TCP scaffolds were fabricated using reticulated polyurethane foam as sacrificial scaffold with a porosity of 70–90 vol %. PCL was coated on sintered porous TCP scaffolds by dipping-drying process. The compressive strength of TCP scaffolds increased significantly after PCL coating. The highest strength of 2.41 MPa at a porosity of 70% was obtained for the TCP scaffold coated with 5% PCL solution. Model protein bovine serum albumin (BSA) was encapsulated efficiently within the PCL coating. The amount of BSA encapsulation was controlled by varying proteins’ composition in the PCL coating. The FTIR analysis confirmed that BSA retained its structural conformation and did not show significant denaturization during PCL coating. The release kinetics in phosphate buffer solution indicated that the protein release was controlled and sustained, and primarily dependant on protein concentration encapsulated in the PCL coating.
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影响因子：
14
作者：
Yuan, Jie;Cui, Lei;Cao, Yilin
通讯作者：
Cao, Yilin
影响因子：
4.9
作者：
Yeo, A.;Rai, B.;Teoh, S. H.
通讯作者：
Teoh, S. H.
DOI：
10.1016/j.msec.2006.12.010
发表时间：
2008-01-01
期刊：
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
影响因子：
--
作者：
Seeley, Zachary;Bandyopadhyay, Amit;Bose, Susmita
通讯作者：
Bose, Susmita
影响因子：
4.9
作者：
Rai, Bina;Oest, Megan E.;Guldberg, Robert E.
通讯作者：
Guldberg, Robert E.
DOI：
10.1002/jbm.1258
发表时间：
2002-03-05
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Ziegler, J;Mayr-Wohlfart, U;Günther, KP
通讯作者：
Günther, KP