A new B-PAn-P system for the detection of bacteria population
A new B-PAn-P system for the detection of bacteria population
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一种用于检测细菌群体的新型 B-PAn-P 系统
DOI：
10.1016/j.snb.2007.02.053
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发表时间：
2007-08
影响因子：
8.4
通讯作者：
He, Fengjiao
中科院分区：
文献类型：
--
作者：
Liu, Zhenhua;Zhang, Lingling;Su, Changwei;Ren, Jiali;He, Fengjiao
提出了一种新的碱基型聚苯胺压电细菌培养系统(B-PAN-P)，用于细菌种群的快速检测。在该系统中，在多通道串联压电石英晶体(MSPQC)系统中，用涂覆了碱性聚苯胺/聚乙烯醇(B-PAN/PVA)复合膜的叉指电极代替钢电极作为探头(B-PAN探头)。该探头直接连接到压电式检测系统。B-PAN/PVA薄膜会与培养介质中细菌生长产生的二氧化碳发生反应，导致薄膜的电学性能发生变化。这种变化可以通过压电检测系统进行灵敏的监测。用该探头代替钢电极的优点是：第一，它对细菌的生长反应更敏感；第二，它不需要MSPQC系统所要求的特殊配方的培养液。就像MSPQC一样，所提出的系统可以用于检测细菌种群。当用于铜绿假单胞菌的定量检测时，频移曲线的频率检测时间与铜绿假单胞菌初始浓度的对数在10~106cfuml−1范围内呈线性关系，检出限为10cfuml−1。与MSPQC系统相比，该系统具有更高的灵敏度，对培养基无特殊要求。
A new base-type polyaniline piezoelectric bacteria culture (B-PAn-P) system for rapid detection of bacteria population was proposed. In this system, interdigital electrode coated with a base-type polyaniline/poly vinyl alcohol (B-PAn/PVA) composite film was used as probe (B-PAn probe) in place of steel electrode in multi-channel series piezoelectric quartz crystal (MSPQC) system. The probe was directly connected to the piezoelectric detection system. B-PAn/PVA film can react with CO2produced by the growth of bacteria in culture medium, which caused the film electrical properties change. This change can be sensitively monitored by piezoelectric detection system. The superiority of using proposed probe instead of steel electrode were: firstly, it was more sensitive to bacteria growth response and secondly, it did not require a specially formulated culture medium which was demanded in MSPQC system. Just like MSPQC, proposed system can be used to detect bacteria population. When used to quantitatively determine Pseudomonas aeruginosa, the frequency detection time (FDT) obtained in frequency shift curve had a linear relationship with the logarithm values of initial concentration of P. aeruginosa in the range of 10 to 106cfuml−1. The detection limit was 10cfuml−1. Compared with MSPQC system, proposed system was more sensitive and no special demand to culture medium.
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DOI：
10.1093/jac/25.suppl_c.41
发表时间：
1990
期刊：
The Journal of antimicrobial chemotherapy
影响因子：
--
作者：
Susannah J. Eykyn;W. Gransden;Ian Phillips
通讯作者：
Susannah J. Eykyn;W. Gransden;Ian Phillips
影响因子：
9.4
作者：
Durmaz, G;Us, T;Akgün, Y
通讯作者：
Akgün, Y
影响因子：
2.2
作者：
F. He;Xiaoqing Zhang;Jiandang Zhou;Zhenhua Liu
通讯作者：
F. He;Xiaoqing Zhang;Jiandang Zhou;Zhenhua Liu
影响因子：
4.5
作者：
T. Oho;T. Tonosaki;K. Isomura;K. Ogura
通讯作者：
T. Oho;T. Tonosaki;K. Isomura;K. Ogura
影响因子：
168.9
作者：
I. Phillips;S. Eykyn;M. Laker
通讯作者：
I. Phillips;S. Eykyn;M. Laker