Scaffold-Type Structure Dental Ceramics with Different Compositions Evaluated through Physicochemical Characteristics and Biosecurity Profiles.
Scaffold-Type Structure Dental Ceramics with Different Compositions Evaluated through Physicochemical Characteristics and Biosecurity Profiles.
复制标题
不同成分支架型牙科陶瓷的理化特性及生物安全性评价。
DOI：
10.3390/ma14092266
复制
发表时间：
2021-04-27
期刊：
影响因子：
--
通讯作者：
Sinescu C
中科院分区：
文献类型：
--
作者：
Fabricky MMC;Gabor AG;Milutinovici RA;Watz CG;Avram Ș;Drăghici G;Mihali CV;Moacă EA;Dehelean CA;Galuscan A;Buzatu R;Duma VF;Negrutiu ML;Sinescu C
基于三维支架的陶瓷结构作为牙体骨替代物的设计和开发已成为再生牙科研究领域的一个热门课题。在这方面，本研究的重点是开发两个支架型结构，从不同的商业牙科陶瓷采用泡沫复制方法。与此同时，该研究强调了新开发的支架的理化特征和生物学特性，与两种传统的用于骨增量的Cerabone®材料相比，通过在刺激后24，48和96 h进行体外Alcohol蓝增殖试验和卵内鸡胚绒毛尿囊膜（CAM）试验。数据显示，新开发的支架表现出与传统Cerabone®增强块相当的结果。在网络孔隙率方面，与Cerabone®颗粒相比，支架显示出更高的孔隙互连性，而在生物安全性方面，所有陶瓷样品对原代人牙龈成纤维细胞（HGF）表现出良好的生物相容性;然而，只有Cerabone®样品在暴露于5和10 µg/mL浓度后诱导HGF细胞增殖。此外，所有供试品均未对血管发育丛产生刺激活性。因此，根据目前的结果，陶瓷支架的初步生物安全概况支持进一步测试其可能的临床牙科应用的高度相关性的有用性。
The design and development of ceramic structures based on 3D scaffolding as dental bone substitutes has become a topic of great interest in the regenerative dentistry research area. In this regard, the present study focuses on the development of two scaffold-type structures obtained from different commercial dental ceramics by employing the foam replication method. At the same time, the study underlines the physicochemical features and the biological profiles of the newly developed scaffolds, compared to two traditional Cerabone® materials used for bone augmentation, by employing both the in vitro Alamar blue proliferation test at 24, 48 and 96 h poststimulation and the in ovo chick chorioallantoic membrane (CAM) assay. The data reveal that the newly developed scaffolds express comparable results with the traditional Cerabone® augmentation masses. In terms of network porosity, the scaffolds show higher pore interconnectivity compared to Cerabone® granules, whereas regarding the biosafety profile, all ceramic samples manifest good biocompatibility on primary human gingival fibroblasts (HGFs); however only the Cerabone® samples induced proliferation of HGF cells following exposure to concentrations of 5 and 10 µg/mL. Additionally, none of the test samples induce irritative activity on the vascular developing plexus. Thus, based on the current results, the preliminary biosecurity profile of ceramic scaffolds supports the usefulness for further testing of high relevance for their possible clinical dental applications.
登录
查看更多内容
DOI：
10.3390/ijerph18073449
发表时间：
2021-03-26
影响因子：
--
作者：
Cattoni F;Chirico L;Merlone A;Manacorda M;Vinci R;Gherlone EF
通讯作者：
Gherlone EF
影响因子：
5.6
作者：
Gao C;Deng Y;Feng P;Mao Z;Li P;Yang B;Deng J;Cao Y;Shuai C;Peng S
通讯作者：
Peng S
影响因子：
14
作者：
Chen, QZZ;Thompson, ID;Boccaccini, AR
通讯作者：
Boccaccini, AR
影响因子：
4.6
作者：
Coelho, Catarina C.;Grenho, Liliana;Fernandes, Maria H.
通讯作者：
Fernandes, Maria H.
影响因子：
3.5
作者：
Kilic, Kerem;Kesim, Bulent;Kesim, Servet
通讯作者：
Kesim, Servet