The Vitamin B1 Metabolism of Staphylococcus aureus Is Controlled at Enzymatic and Transcriptional Levels
The Vitamin B1 Metabolism of Staphylococcus aureus Is Controlled at Enzymatic and Transcriptional Levels
复制标题
金黄色葡萄球菌的维生素 B1 代谢在酶和转录水平上受到控制
作者：
I. Müller;B. Bergmann;M. Groves;I. Couto;L. Amaral;T. Begley;R. D. Walter;C. Wrenger
维生素B1是其活性形式硫胺素焦磷酸盐（TPP），是碳水化合物代谢中几种关键酶的重要辅助因子。哺乳动物必须从它们的饮食中抢救这种重要的营养素，以补充从头合成的不足。在人类致病菌金黄色葡萄球菌中，鉴定出两种参与维生素B1代谢的操纵子。第一操纵子编码II型硫胺素酶（TenA）、4-氨基-5-羟甲基-2-甲基嘧啶激酶（ThiD）、5-（2-羟乙基）-4-甲基噻唑激酶（ThiM）和磷酸硫胺素合酶（ThiE）。第二个操纵子编码磷酸酶、差向异构酶和硫胺素焦磷酸激酶（TPK）。克隆各个操纵子的开放阅读框，重组表达它们相应的蛋白质并进行生化分析。酶的动力学特性以及TPP与体外转录的操纵子RNA的结合表明，维生素B1在S.金黄色葡萄球菌在转录以及酶水平上受到强烈调控。
Vitamin B1 is in its active form thiamine pyrophosphate (TPP), an essential cofactor for several key enzymes in the carbohydrate metabolism. Mammals must salvage this crucial nutrient from their diet in order to complement the deficiency of de novo synthesis. In the human pathogenic bacterium Staphylococcus aureus, two operons were identified which are involved in vitamin B1 metabolism. The first operon encodes for the thiaminase type II (TenA), 4-amino-5-hydroxymethyl-2-methylpyrimidine kinase (ThiD), 5-(2-hydroxyethyl)-4-methylthiazole kinase (ThiM) and thiamine phosphate synthase (ThiE). The second operon encodes a phosphatase, an epimerase and the thiamine pyrophosphokinase (TPK). The open reading frames of the individual operons were cloned, their corresponding proteins were recombinantly expressed and biochemically analysed. The kinetic properties of the enzymes as well as the binding of TPP to the in vitro transcribed RNA of the proposed operons suggest that the vitamin B1 homeostasis in S. aureus is strongly regulated at transcriptional as well as enzymatic levels.
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影响因子：
16.6
作者：
Jurgenson CT;Begley TP;Ealick SE
通讯作者：
Ealick SE
影响因子：
5.7
作者：
Baker, LJ;Dorocke, JA;Timm, DE
通讯作者：
Timm, DE
水动力剪切条件下独立于纤维蛋白原和 IgG 的新型金黄色葡萄球菌-红细胞粘附机制的特征。
DOI：
10.1152/ajpcell.00034.2005
发表时间：
2005
期刊：
American journal of physiology. Cell physiology
影响因子：
--
作者：
Shin,PyongKyun;Pawar,Parag;Konstantopoulos,Konstantinos;Ross,JuliaM
通讯作者：
Ross,JuliaM
影响因子：
14.8
作者：
Jenkins, Amy Haas;Schyns, Ghislain;Begley, Tadhg P.
通讯作者：
Begley, Tadhg P.
影响因子：
8.9
作者：
H. Sauberlich
通讯作者：
H. Sauberlich