Using magnetic resonance imaging to evaluate dendritic cell-based vaccination.
Using magnetic resonance imaging to evaluate dendritic cell-based vaccination.
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DOI：
10.1371/journal.pone.0065318
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Hermans IF
中科院分区：
文献类型：
--
作者：
Ferguson PM;Slocombe A;Tilley RD;Hermans IF
使用负载抗原的树突状细胞疫苗的癌症免疫治疗可以在一些患者中诱导临床反应，但需要进一步优化以释放这种策略在临床中的全部潜力。优化取决于能够监测树突状细胞在体内注射后发生的细胞事件，并确定是否诱导了针对肿瘤的抗原特异性免疫应答。在这里，我们描述了使用磁共振成像（MRI）作为一个简单的，非侵入性的方法来评估疫苗的成功。通过用高磁性铁纳米颗粒装载树突状细胞，可以评估注射的细胞是否流向淋巴结。也可以通过评估连续几轮抗原负载的树突状细胞的迁移来确定抗原特异性应答是否启动;面对成功引发的细胞毒性应答，大量抗原负载的细胞在到达淋巴结的途中被根除，而没有抗原的细胞可以未经检查地到达淋巴结。也可以通过简单监测引流淋巴结大小的增加来验证疫苗诱导的应答的诱导，引流淋巴结大小的增加是疫苗诱导的淋巴细胞捕获的结果，这是一种抗原特异性应答，随着重复接种变得更加明显。总体而言，这些MRI技术可以提供有关疫苗接种策略的有用的早期反馈，并且还可以用于决策，以在治疗早期从无应答者中选择应答者。
Cancer immunotherapy with antigen-loaded dendritic cell-based vaccines can induce clinical responses in some patients, but further optimization is required to unlock the full potential of this strategy in the clinic. Optimization is dependent on being able to monitor the cellular events that take place once the dendritic cells have been injected in vivo, and to establish whether antigen-specific immune responses to the tumour have been induced. Here we describe the use of magnetic resonance imaging (MRI) as a simple, non-invasive approach to evaluate vaccine success. By loading the dendritic cells with highly magnetic iron nanoparticles it is possible to assess whether the injected cells drain to the lymph nodes. It is also possible to establish whether an antigen-specific response is initiated by assessing migration of successive rounds of antigen-loaded dendritic cells; in the face of a successfully primed cytotoxic response, the bulk of antigen-loaded cells are eradicated on-route to the node, whereas cells without antigen can reach the node unchecked. It is also possible to verify the induction of a vaccine-induced response by simply monitoring increases in draining lymph node size as a consequence of vaccine-induced lymphocyte trapping, which is an antigen-specific response that becomes more pronounced with repeated vaccination. Overall, these MRI techniques can provide useful early feedback on vaccination strategies, and could also be used in decision making to select responders from non-responders early in therapy.
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影响因子：
15.3
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