Characterization of MSB synapses in dissociated hippocampal culture with simultaneous pre- and postsynaptic live microscopy.
Characterization of MSB synapses in dissociated hippocampal culture with simultaneous pre- and postsynaptic live microscopy.
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DOI：
10.1371/journal.pone.0026478
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Phillips GR
中科院分区：
文献类型：
--
作者：
Reilly JE;Hanson HH;Fernández-Monreal M;Wearne SL;Hof PR;Phillips GR
多突触突触(MSB)是指与多个突触后伙伴接触的突触前突触。虽然已经用电子显微镜(EM)等静态成像技术研究了MSB突触，但还没有直接评估单个MSB突触的动力学。众所周知，MSB突触的数量随着突触发生和可塑性的增加而增加，但单个MSB突触的形成、行为和命运在很大程度上仍不清楚。为了解决这个问题，我们开发了一种对MSB突触进行实时成像的方法，以便随着时间的推移直接观察它们。用时移共聚焦显微镜观察了与VAMP2-DsRed标记的Boutons接触的GFP填充的树突，我们每小时记录一次MSB及其接触的脊椎超过15个小时。我们的活显微镜显示，与接触单个突触小环(SSB)的脊柱相比，接触MSB的脊柱表现出更高的动态行为。这些结果与MSB在突触发育和可塑性中起中介作用的观点是一致的。
Multisynaptic boutons (MSBs) are presynaptic boutons in contact with multiple postsynaptic partners. Although MSB synapses have been studied with static imaging techniques such as electron microscopy (EM), the dynamics of individual MSB synapses have not been directly evaluated. It is known that the number of MSB synapses increases with synaptogenesis and plasticity but the formation, behavior, and fate of individual MSB synapses remains largely unknown. To address this, we developed a means of live imaging MSB synapses to observe them directly over time. With time lapse confocal microscopy of GFP-filled dendrites in contact with VAMP2-DsRed-labeled boutons, we recorded both MSBs and their contacting spines hourly over 15 or more hours. Our live microscopy showed that, compared to spines contacting single synaptic boutons (SSBs), MSB-contacting spines exhibit elevated dynamic behavior. These results are consistent with the idea that MSBs serve as intermediates in synaptic development and plasticity.
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DOI：
10.1111/j.1460-9568.2010.07386.x
发表时间：
2010-09
期刊：
The European journal of neuroscience
影响因子：
--
作者：
Fernández-Monreal M;Oung T;Hanson HH;O'Leary R;Janssen WG;Dolios G;Wang R;Phillips GR
通讯作者：
Phillips GR
影响因子：
16.2
作者：
Holtmaat, AJGD;Trachtenberg, JT;Svoboda, K
通讯作者：
Svoboda, K
影响因子：
5.3
作者：
Korkotian, E;Segal, M
通讯作者：
Segal, M
影响因子：
14.8
作者：
Rodriguez, Alfredo;Ehlenberger, Douglas B.;Wearne, Susan L.
通讯作者：
Wearne, Susan L.
影响因子：
2.5
作者：
Nicholson, Daniel A.;Geinisnian, Yuri
通讯作者：
Geinisnian, Yuri