Significant type I and type III collagen production from human periodontal ligament fibroblasts in 3D peptide scaffolds without extra growth factors.
Significant type I and type III collagen production from human periodontal ligament fibroblasts in 3D peptide scaffolds without extra growth factors.
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DOI：
10.1371/journal.pone.0010305
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发表时间：
2010-04-22
期刊：
影响因子：
3.7
通讯作者：
Zhang S
中科院分区：
文献类型：
--
作者：
Kumada Y;Zhang S
本文报道了两种用于牙周膜成纤维细胞的多肽支架的研制。该支架由一种纯的自组装多肽支架RADA16通过直接偶联到短的生物活性基序组成。这些基序是两个单位的RGD结合序列PRG(PRGDSGYRGDS)和层粘连蛋白细胞黏附基序PDS(PDSGR)。RGD和层粘连蛋白已经被证明可以促进特定的生物活性，包括牙周膜成纤维细胞的黏附、增殖和蛋白质的产生。与纯RADA16多肽支架相比，我们发现这些设计的多肽支架显著促进人牙周膜成纤维细胞的增殖和向支架内迁移(∼为300微米/两周)。此外，这些多肽支架显着刺激牙周膜成纤维细胞产生细胞外基质蛋白，而不使用额外的生长因子。免疫荧光图像清楚地显示，多肽支架几乎完全被牙周膜的主要蛋白质成分--I型和III型胶原所覆盖。我们的结果表明，这些设计者自组装的多肽纳米纤维支架可能有助于促进伤口愈合，特别是牙周韧带组织的再生。
We here report the development of two peptide scaffolds designed for periodontal ligament fibroblasts. The scaffolds consist of one of the pure self-assembling peptide scaffolds RADA16 through direct coupling to short biologically active motifs. The motifs are 2-unit RGD binding sequence PRG (PRGDSGYRGDS) and laminin cell adhesion motif PDS (PDSGR). RGD and laminin have been previously shown to promote specific biological activities including periodontal ligament fibroblasts adhesion, proliferation and protein production. Compared to the pure RADA16 peptide scaffold, we here show that these designer peptide scaffolds significantly promote human periodontal ligament fibroblasts to proliferate and migrate into the scaffolds (for ∼300 µm/two weeks). Moreover these peptide scaffolds significantly stimulated periodontal ligament fibroblasts to produce extracellular matrix proteins without using extra additional growth factors. Immunofluorescent images clearly demonstrated that the peptide scaffolds were almost completely covered with type I and type III collagens which were main protein components of periodontal ligament. Our results suggest that these designer self-assembling peptide nanofiber scaffolds may be useful for promoting wound healing and especially periodontal ligament tissue regeneration.
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影响因子：
64.5
作者：
Choquet, D;Felsenfeld, DP;Sheetz, MP
通讯作者：
Sheetz, MP
影响因子：
6.7
作者：
GANTES, B;MARTIN, M;EGELBERG, J
通讯作者：
EGELBERG, J
影响因子：
7.6
作者：
KARIMBUX, NY;NISHIMURA, I
通讯作者：
NISHIMURA, I
影响因子：
3.5
作者：
Murakami, S;Takayama, S;Okada, H
通讯作者：
Okada, H
纳米神经编织：用于大脑修复和轴突再生以及视力功能恢复的肽纳米纤维支架
DOI：
10.1073/pnas.0600559103
发表时间：
2006-03-28
影响因子：
11.1
作者：
Ellis-Behnke, RG;Liang, YX;Schneider, GE
通讯作者：
Schneider, GE