Analysis of the chromosome complement in outbred mouse sperm fertilizing in vitro.
Analysis of the chromosome complement in outbred mouse sperm fertilizing in vitro.
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远交小鼠精子体外受精的染色体补体分析。
DOI：
10.1002/mrd.1120220108
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Martin-DeLeon,PA
中科院分区：
文献类型：
--
作者：
Martin-DeLeon,PA
对来自随机繁殖的ICR供体的小鼠精子进行了体外受精(IVF)后的第一卵裂中期染色体补体分析。精子的老化是因为捐献发生在平均31天内，无论是在最后一次交配后，还是在处女雄性精子到达动物设施时。在22名10-26周龄的性成熟男性的总共598个精子补体中，有一个二倍体补体(0.17%)。在338个补体中，超单倍体和结构畸变率分别为4.4%和3.6%，总频率为8.0%。超单倍体补体包括n+1、n+2、n+3和n+7个，结构异常为染色体类型，包括大小片段和可能的易位。这是在生理条件下从雄性小鼠身上获得并在体外或体内受精的小鼠精子中精子染色体异常频率最高的报告。精子老化、紧张和/或技术差异可能是导致这种高频率的因素之一。由于体外受精后超单倍体和结构异常的发生率为8.0%，与已报道的人类精子相似，因此体外受精小鼠体外受精系统可能成为研究人类精子染色体异常起源的有用模型。
The chromosome complements in a population of mouse sperm from random‐bred ICR donors were analyzed at first‐cleavage metaphase after in vitro fertilization (IVF) of oocytes from females of the same strain. The sperm were aged as donations occurred within an average of 31 days, either since last mating or at arrival at the animal facility in the case of virgin males. Of a total of 598 sperm complements studied from 22 sexually mature males aged 10–26 weeks old, there was one diploid complement (0.17%). The frequencies of hyperhaploidy and structural aberrations that were studied in 338 complements were 4.4% and 3.6%, respectively, giving an overall frequency of 8.0%. The hyperhaploid complements consisted of n + 1, n + 2, n + 3, and n + 7 counts, while the structural abnormalities were of the chromosome type and included large and small fragments and a possible translocation. This is the highest frequency of sperm chromosome abnormalities reported for mouse sperm obtained from males under physiological conditions and fertilized in vitro or in vivo. Sperm aging, strain, and/or technique differences are among the factors that may be responsible for this high frequency. Since the 8.0% frequency of hyperhaploidy and structural abnormalities is similar to the frequency reported for human sperm after IVF, the outbred murine in vitro fertilization system may be a useful model to study the origin of human sperm chromosome abnormalities.
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DOI：
10.1530/jrf.0.0620385
发表时间：
1981-01-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
KRZANOWSKA, H
通讯作者：
KRZANOWSKA, H
体外受精的多原核人卵母细胞的染色体分析。
影响因子：
6.7
作者：
E. Rudak;E. Rudak;Jehoshua Dor;Jehoshua Dor;S. Mashiach;S. Mashiach;Laslo Nebel;Laslo Nebel;Boleslav Goldman;Boleslav Goldman
通讯作者：
Boleslav Goldman
人类体外受精的染色体研究
影响因子：
5.3
作者：
R. Angell;A. Templeton;R. Aitken
通讯作者：
R. Aitken
小鼠同源盒基因在精母细胞和胚胎中表达。
影响因子：
56.9
作者：
M. Rubin;L. E. Toth;M. Patel;P. D’Eustachio;M. Nguyen
通讯作者：
M. Nguyen
一种使用无透明带仓鼠卵子分析人类精子染色体的改进、高效方法。
影响因子：
9.8
作者：
Y. Kamiguchi;K. Mikamo
通讯作者：
K. Mikamo