The optokinetic response as a quantitative measure of visual acuity in zebrafish.
The optokinetic response as a quantitative measure of visual acuity in zebrafish.
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DOI：
10.3791/50832
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发表时间：
2013-10-09
期刊：
影响因子：
--
通讯作者：
Dehkordi MI
中科院分区：
文献类型：
--
作者：
Cameron DJ;Rassamdana F;Tam P;Dang K;Yanez C;Ghaemmaghami S;Dehkordi MI
斑马鱼是一种经过验证的视觉研究模型，然而许多早期的方法通常集中在幼鱼或表现出简单的反应。最近，斑马鱼的成年视觉行为已经引起了人们的兴趣，但测量特定反应的方法是新的。为了解决这一差距，我们着手开发一种方法来重复和准确地利用视动反应（OKR）来测量成年斑马鱼的视敏度。在这里，我们表明，成年斑马鱼的视力可以测量，包括双眼和单眼的视力。由于鱼在手术过程中不会受到伤害，因此可以在短时间或长时间内测量和比较视力。这里描述的视力测量也可以快速完成，从而实现高通量并在需要时进行额外的视觉程序。这种类型的分析有助于药物干预研究或疾病进展的调查。
Zebrafish are a proven model for vision research, however many of the earlier methods generally focused on larval fish or demonstrated a simple response. More recently adult visual behavior in zebrafish has become of interest, but methods to measure specific responses are new coming. To address this gap, we set out to develop a methodology to repeatedly and accurately utilize the optokinetic response (OKR) to measure visual acuity in adult zebrafish. Here we show that the adult zebrafish's visual acuity can be measured, including both binocular and monocular acuities. Because the fish is not harmed during the procedure, the visual acuity can be measured and compared over short or long periods of time. The visual acuity measurements described here can also be done quickly allowing for high throughput and for additional visual procedures if desired. This type of analysis is conducive to drug intervention studies or investigations of disease progression.
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DOI：
10.1101/pdb.prot4961
发表时间：
2008-03-01
期刊：
CSH protocols
影响因子：
--
作者：
Hodel, Corinne;Neuhauss, Stephan C F
通讯作者：
Neuhauss, Stephan C F
影响因子：
5.3
作者：
Maaswinkel, H;Mason, B;Li, L
通讯作者：
Li, L
影响因子：
4.5
作者：
Veth KN;Willer JR;Collery RF;Gray MP;Willer GB;Wagner DS;Mullins MC;Udvadia AJ;Smith RS;John SW;Gregg RG;Link BA
通讯作者：
Link BA
影响因子：
3
作者：
Mueller, Kaspar P.;Neuhauss, Stephan C. F.
通讯作者：
Neuhauss, Stephan C. F.
DOI：
10.1073/pnas.92.23.10545
发表时间：
1995-11-07
影响因子：
11.1
作者：
BROCKERHOFF, SE;HURLEY, JB;DOWLING, JE
通讯作者：
DOWLING, JE