Chick cranial neural crest cells use progressive polarity refinement, not contact inhibition of locomotion, to guide their migration.
Chick cranial neural crest cells use progressive polarity refinement, not contact inhibition of locomotion, to guide their migration.
复制标题
雏鸡颅神经嵴细胞使用渐进极性细化，而不是运动的接触抑制来引导其迁移。
DOI：
10.1016/j.ydbio.2018.02.016
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发表时间：
2018-12-01
影响因子：
2.7
通讯作者：
Weiner OD
中科院分区：
文献类型：
--
作者：
Genuth MA;Allen CDC;Mikawa T;Weiner OD
为了进行定向移动，细胞可以偏置突起的生成或在随机生成的突起中进行选择。在这里，我们使用小鸡鳃弓定向神经嵴细胞的三维双光子成像来探索这些机制如何促进定向运动，是一部分细胞还是大多数细胞在运动过程中极化，以及不同类型的突起如何相互关联。我们发现，与非洲爪蟾相反，整个流中的细胞沿着整体流运动的方向在形态上极化，并且不表现出运动的接触抑制。相反，鸡的神经嵴细胞表现出一种逐渐锐化的形态极性程序。神经嵴细胞在丝状足的产生和寿命上有较弱的空间偏差。丝状突起的局部爆发先于较大突起的产生。这些较大的突起比丝状足在空间上更偏倚，而对运动有生产力的突起子集是所有突起中最极化的。方向而不是位置是选择用于细胞引导的突起的最佳关联。这种渐进式极性优化策略可以使神经嵴细胞有效地探索其环境，并在面对噪声引导信号时准确迁移。
To move directionally, cells can bias the generation of protrusions or select among randomly generated protrusions. Here we use 3D two-photon imaging of chick branchial arch 2 directed neural crest cells to probe how these mechanisms contribute to directed movement, whether a subset or the majority of cells polarize during movement, and how the different classes of protrusions relate to one another. We find that, in contrast to Xenopus, cells throughout the stream are morphologically polarized along the direction of overall stream movement and do not exhibit contact inhibition of locomotion. Instead chick neural crest cells display a progressive sharpening of the morphological polarity program. Neural crest cells have weak spatial biases in filopodia generation and lifetime. Local bursts of filopodial generation precede the generation of larger protrusions. These larger protrusions are more spatially biased than the filopodia, and the subset of protrusions that are productive for motility are the most polarized of all. Orientation rather than position is the best correlate of the protrusions that are selected for cell guidance. This progressive polarity refinement strategy may enable neural crest cells to efficiently explore their environment and migrate accurately in the face of noisy guidance cues.
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DOI：
10.1146/annurev-cellbio-100616-060739
发表时间：
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影响因子：
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