Unexpected diversity of cnidarian integrins: expression during coral gastrulation.
Unexpected diversity of cnidarian integrins: expression during coral gastrulation.
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DOI：
10.1186/1471-2148-8-136
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发表时间：
2008-05-09
影响因子：
3.4
通讯作者：
Miller DJ
中科院分区：
文献类型：
--
作者：
Knack BA;Iguchi A;Shinzato C;Hayward DC;Ball EE;Miller DJ
通过细胞表面受体整合素家族介导的粘附对整个后生动物的早期发育至关重要，在原肠胚聚集和伸展运动过程中，在细胞-细胞外基质的粘附和钙粘蛋白活性的调节中起着关键作用。秀丽隐杆线虫具有1个β和2个α整合素，可能反映了其祖先的整合素补体。为了验证这一假设，需要对珊瑚虫针孔动物（如千孔虫Acropora millepora）的整合素库进行研究，并可能为这些分子的原始作用提供见解。在鹿角菌中鉴定出两个新的整合素。AmItgα1表现出缺乏i结构域的α整合素的特征，但系统发育分析没有明确表明其可能的结合特异性。AmItgβ2在8位和9位缺乏一致的半胱氨酸残基，但在其他方面是一个典型的β整联素。原位杂交结果显示，在原肠胚形成过程中，AmItgα1、AmItgβ1和AmItgβ2在内胚层中表达。第二种珊瑚虫，海葵Nematostella vectensis，根据其基因组序列，具有至少四个β整合素，两个类似于AmItgβ1，两个类似于AmItgβ2，以及至少三个α整合素。在两个方面，逐点数据不符合预期。首先，刺胞整合素库比预测的要复杂：在Acropora中至少有2个βs，在Nematostella中至少有3个αs和4个βs。其次，尽管双边αs分解成与含有rgd或层粘连蛋白型配体特异性相对应的良好支持基团，但已知的刺胞体αs与这些不同。在Acropora的早期发育过程中，这三种已知整合素的表达模式与两栖动物和棘皮动物的整合素相似。
Adhesion mediated through the integrin family of cell surface receptors is central to early development throughout the Metazoa, playing key roles in cell-extra cellular matrix adhesion and modulation of cadherin activity during the convergence and extension movements of gastrulation. It has been suggested that Caenorhabditis elegans, which has a single β and two α integrins, might reflect the ancestral integrin complement. Investigation of the integrin repertoire of anthozoan cnidarians such as the coral Acropora millepora is required to test this hypothesis and may provide insights into the original roles of these molecules. Two novel integrins were identified in Acropora. AmItgα1 shows features characteristic of α integrins lacking an I-domain, but phylogenetic analysis gives no clear indication of its likely binding specificity. AmItgβ2 lacks consensus cysteine residues at positions 8 and 9, but is otherwise a typical β integrin. In situ hybridization revealed that AmItgα1, AmItgβ1, and AmItgβ2 are expressed in the presumptive endoderm during gastrulation. A second anthozoan, the sea anemone Nematostella vectensis, has at least four β integrins, two resembling AmItgβ1 and two like AmItgβ2, and at least three α integrins, based on its genomic sequence. In two respects, the cnidarian data do not fit expectations. First, the cnidarian integrin repertoire is more complex than predicted: at least two βs in Acropora, and at least three αs and four βs in Nematostella. Second, whereas the bilaterian αs resolve into well-supported groups corresponding to those specific for RGD-containing or laminin-type ligands, the known cnidarian αs are distinct from these. During early development in Acropora, the expression patterns of the three known integrins parallel those of amphibian and echinoderm integrins.
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作者：
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通讯作者：
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