Tools to study pathogen-host interactions in bats.
Tools to study pathogen-host interactions in bats.
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DOI：
10.1016/j.virusres.2018.02.013
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发表时间：
2018-03-15
期刊：
影响因子：
5
通讯作者：
Baker ML
中科院分区：
文献类型：
--
作者：
Banerjee A;Misra V;Schountz T;Baker ML
蝙蝠是新出现的人畜共患病毒的重要储存宿主。在蝙蝠中检测到的病毒很难用传统的细胞系分离出来。蝙蝠细胞系提供了重要的工具，剖析宿主病原体的相互作用。对蝙蝠的免疫细胞数量及其反应知之甚少。共享试剂和细胞系将加速研究和病毒发现。蝙蝠是各种新兴病毒的天然宿主，这些病毒在人类和家畜中引起重大疾病，但很少在蝙蝠中引起临床疾病。蝙蝠与病毒的共同进化历史被假设为塑造了蝙蝠与病毒的关系，使两者能够平衡存在。近年来，由于易感蝙蝠细胞系的发展，在了解蝙蝠病毒相互作用和分离蝙蝠传播病毒方面的进展加快了。在蝙蝠体内检测到与严重急性呼吸综合征冠状病毒（SARS-CoV）相似的病毒序列，并从蝙蝠体内分离出马尔堡病毒等丝状病毒，为蝙蝠作为天然宿主的作用提供了明确的证据。虽然病毒可以很容易地检测到蝙蝠使用分子方法，病毒分离是更具挑战性。使用来自非储库物种的传统培养系统的限制之一是细胞类型和培养条件可能不适于分离蝙蝠传播的病毒。因此，需要开发对应于不同细胞类型（包括较少代表的细胞类型如免疫细胞）的另外的蝙蝠细胞系，并在更生理相关的条件下培养它们以研究病毒宿主相互作用和用于病毒分离。在这篇综述中，我们强调了目前在蝙蝠细胞系系统中了解蝙蝠病毒相互作用的进展，以及与细胞系相关的一些挑战和限制。未来的方向，以解决这些挑战，以更好地了解宿主-病原体相互作用，在这些有趣的哺乳动物也进行了讨论，不仅在有关病毒，但也包括细菌和真菌的蝙蝠携带的其他病原体。
Bats are important reservoir hosts for emerging zoonotic viruses. Viruses detected in bats are difficult to isolate using traditional cell lines. Bat cell lines provide critical tools to dissect host pathogen interactions. Little is known about immune cell populations and their responses in bats. Sharing reagents and cell lines will accelerate research and virus discovery. Bats are natural reservoirs for a variety of emerging viruses that cause significant disease in humans and domestic animals yet rarely cause clinical disease in bats. The co-evolutionary history of bats with viruses has been hypothesized to have shaped the bat-virus relationship, allowing both to exist in equilibrium. Progress in understanding bat-virus interactions and the isolation of bat-borne viruses has been accelerated in recent years by the development of susceptible bat cell lines. Viral sequences similar to severe acute respiratory syndrome corona virus (SARS-CoV) have been detected in bats, and filoviruses such as Marburg virus have been isolated from bats, providing definitive evidence for the role of bats as the natural host reservoir. Although viruses can be readily detected in bats using molecular approaches, virus isolation is far more challenging. One of the limitations in using traditional culture systems from non-reservoir species is that cell types and culture conditions may not be compatible for isolation of bat-borne viruses. There is, therefore, a need to develop additional bat cell lines that correspond to different cell types, including less represented cell types such as immune cells, and culture them under more physiologically relevant conditions to study virus host interactions and for virus isolation. In this review, we highlight the current progress in understanding bat-virus interactions in bat cell line systems and some of the challenges and limitations associated with cell lines. Future directions to address some of these challenges to better understand host-pathogen interactions in these intriguing mammals are also discussed, not only in relation to viruses but also other pathogens carried by bats including bacteria and fungi.
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影响因子：
3.7
作者：
Crameri G;Todd S;Grimley S;McEachern JA;Marsh GA;Smith C;Tachedjian M;De Jong C;Virtue ER;Yu M;Bulach D;Liu JP;Michalski WP;Middleton D;Field HE;Wang LF
通讯作者：
Wang LF
影响因子：
3.7
作者：
Alves, Davi M. C. C.;Terribile, Levi C.;Brito, Daniel
通讯作者：
Brito, Daniel
影响因子：
3.7
作者：
Eckerle I;Ehlen L;Kallies R;Wollny R;Corman VM;Cottontail VM;Tschapka M;Oppong S;Drosten C;Müller MA
通讯作者：
Müller MA
DOI：
10.3390/v4050889
发表时间：
2012-05
期刊：
Viruses
影响因子：
--
作者：
Jordan I;Munster VJ;Sandig V
通讯作者：
Sandig V
DOI：
10.4269/ajtmh.1963.12.640
发表时间：
1963-01-01
影响因子：
3.3
作者：
DOWNS, WG;GREENHALGH, AH;AITKEN, THG
通讯作者：
AITKEN, THG