Authentication of the R06E fruit bat cell line.

Authentication of the R06E fruit bat cell line.
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DOI:
10.3390/v4050889
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发表时间:
2012-05
期刊:
Viruses
影响因子:
--
通讯作者:
Sandig V
Sandig V
中科院分区:
其他
文献类型:
--
作者:
Jordan I;Munster VJ;Sandig V

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果蝠和食虫蝙蝠被认为是各种传染病的天然宿主。包括成功分离出传染性病毒在内的多项证据表明,马尔堡病毒和拉文病毒在埃及鲁塞特(Rousettus aegyptiacus)的菌落中发现了一个主要宿主。为了促进病毒-储库宿主相互作用的分子研究和从环境样品中分离病毒,我们从这种动物的原代细胞中建立了细胞系。这些细胞系被提供给几个实验室,直到我们意识到其中一个培养物中发生了Vero细胞污染。在这里,我们描述了一个通用的诊断程序,用于识别跨物种污染的重点是Vero和Rousettus细胞系,并总结了新发现的特性的细胞系,可能涉及到病原体的发现。
Fruit bats and insectivorous bats are believed to provide a natural reservoir for a wide variety of infectious diseases. Several lines of evidence, including the successful isolation of infectious viruses, indicate that Marburg virus and Ravn virus have found a major reservoir in colonies of the Egyptian rousette (Rousettus aegyptiacus). To facilitate molecular studies on virus-reservoir host interactions and isolation of viruses from environmental samples, we established cell lines from primary cells of this animal. The cell lines were given to several laboratories until we realized that a contamination with Vero cells in one of the cultures had occurred. Here we describe a general diagnostic procedure for identification of cross-species contamination with the focus on Vero and Rousettus cell lines, and summarize newly discovered properties of the cell lines that may pertain to pathogen discovery.
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