Early development of the thymus in Xenopus laevis.
Early development of the thymus in Xenopus laevis.
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DOI：
10.1002/dvdy.23905
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发表时间：
2013-02
影响因子：
2.5
通讯作者：
Saint-Jeannet, Jean-Pierre
中科院分区：
文献类型：
--
作者：
Lee, Young-Hoon;Williams, Allison;Hong, Chang-Soo;You, Youngjae;Senoo, Makoto;Saint-Jeannet, Jean-Pierre
虽然非洲爪哇一直是免疫系统比较和发育研究的首选模型，但对胸腺的器官发生知之甚少，胸腺是脊椎动物的主要淋巴器官。在这里，我们检测了三个与咽腺发育相关的转录因子的表达，即gcm2、hoxa3和FOXN1，并评估了神经脊对胸腺发育的贡献。在大多数物种中，Hoxa3在第三咽囊内胚层表达，在那里它指导胸腺的形成。在非洲爪蛙中，胸腺原基来自第二咽囊内胚层，它是HOXA3阴性的，这表明在青蛙中调节胸腺形成的机制不同。与其他物种不同的是，FOXN1在非洲爪哇的咽囊上皮中没有检测到，而是在胸腺上皮细胞开始分化并表达MHC II类分子时开始表达。通过移植实验，我们发现，尽管胸腺原基中充斥着神经脊细胞，但它们并不是青蛙胸腺器官的特化和初始发育所必需的，也不是T细胞分化所必需的。这些研究为非洲爪哇早期胸腺发育提供了新的信息，并突出了非洲爪哇有别于其他生物的一些特征。
Although Xenopus laevis has been a model of choice for comparative and developmental studies of the immune system, little is known about organogenesis of the thymus, a primary lymphoid organ in vertebrates. Here we examined the expression of three transcription factors that have been functionally associated with pharyngeal gland development, gcm2, hoxa3 and foxn1, and evaluated the neural crest contribution to thymus development. In most species Hoxa3 is expressed in the third pharyngeal pouch endoderm where it directs thymus formation. In Xenopus, the thymus primordium is derived from the second pharyngeal pouch endoderm, which is hoxa3-negative, suggesting that a different mechanism regulates thymus formation in frogs. Unlike other species foxn1 is not detected in the epithelium of the pharyngeal pouch in Xenopus, rather, its expression is initiated as thymic epithelial cell starts to differentiate and express MHC class II molecules. Using transplantation experiments we show that while neural crest cells populate the thymus primordia, they are not required for the specification and initial development of this organ or for T cell differentiation in frogs. These studies provide novel information on early thymus development in Xenopus, and highlight a number of features that distinguish Xenopus from other organisms.
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影响因子：
64.8
作者：
HUNT, P;GULISANO, M;KRUMLAUF, R
通讯作者：
KRUMLAUF, R
DOI：
10.1073/pnas.1005129107
发表时间：
2010-06-08
影响因子：
11.1
作者：
Chen, Lizhen;Zhao, Peng;Manley, Nancy R.
通讯作者：
Manley, Nancy R.
DOI：
10.1073/pnas.95.21.12364
发表时间：
1998-10-13
影响因子：
11.1
作者：
Kim, JS;Jones, BW;Anderson, DJ
通讯作者：
Anderson, DJ
DOI：
10.1073/pnas.93.12.5742
发表时间：
1996-06-11
影响因子：
11.1
作者：
Blackburn, CC;Augustine, CL;Morahan, G
通讯作者：
Morahan, G
影响因子：
56.9
作者：
BOCKMAN, DE;KIRBY, ML
通讯作者：
KIRBY, ML