The potential contribution of miRNA-200-3p to the fatty acid metabolism by regulating AjEHHADH during aestivation in sea cucumber.
The potential contribution of miRNA-200-3p to the fatty acid metabolism by regulating AjEHHADH during aestivation in sea cucumber.
复制标题
miRNA-200-3p 通过调节 AjEHHADH 对海参夏眠期间脂肪酸代谢的潜在贡献
作者：
Chen M;Wang S;Li X;Storey KB;Zhang X
海参（Apostichopus japonicus）是近年来研究海洋无脊椎动物环境诱导睡眠的良好模式生物。在本研究中，我们假设miRNA-200-3p可能有助于在睡眠过程中建立快速的生物控制来调节脂肪酸代谢。过氧化物酶体双功能酶（EHHADH）是经典的过氧化物酶体脂肪酸β-氧化途径的重要参与者，我们分析了经过长期饲养的海参肠道中EHHADH的相对mRNA转录本和蛋白表达。与非休眠对照组相比，深度休眠期间肠内EHHADH mRNA转录本和蛋白表达量均显著降低。AjEHHADH的3 ' UTR分析显示存在miR-200-3p的保守结合位点。miR-200-3p水平与肠内EHHADH mRNA转录本和蛋白水平呈负相关，提示miR-200-3p可能通过诱导AjEHHADH mRNA降解直接靶向AjEHHADH，进一步的双荧光素酶报告基因实验验证了miRNA-200-3p在调节AjEHHADH中的预测作用。为了进一步了解它们的调控机制，我们进行了体内功能实验。在海参中过表达miR-200-3p可显著降低AjEHHADH mRNA和蛋白的表达水平。综上所述，这些发现表明miRNA-200-3p可能通过调节海参在冬眠期间的AjEHHADH来参与脂肪酸代谢。
The sea cucumber (Apostichopus japonicus) has become a good model organism for studying environmentally-induced aestivation by a marine invertebrate more recently. In the present study, we hypothesized that miRNA-200-3p may contribute to establish rapid biological control to regulate fatty acid metabolism during a estivation. The peroxisomal bi-functional enzyme (EHHADH) is a crucial participant of the classical peroxisomal fatty acid β-oxidation pathway, the relative mRNA transcripts and protein expressions of EHHADH were analyzed in intestine from sea cucumbers experienced long-term aestivation. Both mRNA transcripts and protein expressions of EHHADH in intestine decreased significantly during deep-aestivation as compared with non-aestivation controls. Analysis of the 3′ UTR of AjEHHADH showed the presence of a conserved binding site for miR-200-3p. Level of miR-200-3p showed an inverse correlation with EHHADH mRNA transcripts and protein levels in intestine, implicating miR-200-3p may directly targeted AjEHHADH by inducing the degradation of AjEHHADH mRNA in the aestivating sea cucumber, further dual-luciferase reporter assay validated the predicted role of miRNA-200-3p in regulating AjEHHADH. In order to further understand their regulatory mechanism, we conducted the functional experiment in vivo. The overexpression of miR-200-3p in sea cucumber significantly decreased mRNA and protein expression levels of AjEHHADH. Taken together, these findings suggested the potential contribution of miRNA-200-3p to the fatty acid metabolism by regulating AjEHHADH during aestivation in sea cucumber.
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DOI：
10.1016/j.bbagrm.2008.07.011
发表时间：
2008-10-01
影响因子：
4.7
作者：
Morin, Pier Jr.;Dubuc, Adrian;Storey, Kenneth B.
通讯作者：
Storey, Kenneth B.
影响因子：
15.9
作者：
TONSGARD, JH;GETZ, GS
通讯作者：
GETZ, GS
miRNA-target嵌合体揭示了miRNA 3'-end配对，这是Argonaute目标特异性的主要决定因素。
影响因子：
16.6
作者：
Moore MJ;Scheel TK;Luna JM;Park CY;Fak JJ;Nishiuchi E;Rice CM;Darnell RB
通讯作者：
Darnell RB
影响因子：
9.5
作者：
Biggar, Kyle K.;Kornfeld, Samantha F.;Maistrovski, Yulia;Storey, Kenneth B.
通讯作者：
Storey, Kenneth B.
影响因子：
9.5
作者：
Kornfeld, Samantha F.;Biggar, Kyle K.;Storey, Kenneth B.
通讯作者：
Storey, Kenneth B.