Effects of weaning on intestinal crypt epithelial cells in piglets.
Effects of weaning on intestinal crypt epithelial cells in piglets.
复制标题
断奶对仔猪肠隐窝上皮细胞的影响
DOI：
10.1038/srep36939
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发表时间：
2016-11-10
影响因子：
4.6
通讯作者：
Yin Y
中科院分区：
文献类型：
--
作者：
Yang H;Xiong X;Wang X;Li T;Yin Y
断奶后，仔猪隐窝中的肠上皮细胞会增殖。然而，其根本机制尚不清楚。我们检查了 8 窝中的 40 头仔猪（每窝 5 头仔猪），这些仔猪在 14 日龄断奶，并从每窝中随机抽取 1 头仔猪进行仔细研究。基于扩张肠囊法，我们在断奶后第0、1、3、5和7天从空肠中部分离隐窝上皮细胞。使用同量异位标签进行相对和绝对定量或蛋白质印迹分析蛋白质表达。与细胞周期、细胞大分子复合物亚基的组织、细胞大分子定位、高尔基体囊泡运输、脂肪酸代谢、氧化磷酸化和翻译起始相关的蛋白质主要下调，而与糖酵解、细胞周期停滞、蛋白质分解代谢和细胞氨基酸代谢相关的蛋白质主要上调。 mTOR 信号通路中活性蛋白的数量通常会随着时间的推移而减少。这些结果表明断奶影响能量代谢、细胞大分子组织和定位以及蛋白质代谢，从而影响断奶仔猪肠上皮细胞的增殖。此外，这些细胞过程可能是由该信号通路控制的。
Intestinal epithelial cells in the crypt proliferate in piglets in response to weaning. However, the underlying mechanism has been unclear. We examined 40 piglets from eight litters (five piglets per litter) that were weaned at the age of 14 d, and one piglet from each litter was randomly selected for closer investigation. Based on the distended intestinal sac method, we isolated crypt epithelial cells from the mid-jejunum on Days 0, 1, 3, 5, and 7 post-weaning. Protein expression was analyzed using either isobaric tags for relative and absolute quantification or western blotting. Proteins related to the cell cycle, organization of the cellular macromolecular complex subunit, localization of cellular macromolecules, Golgi vesicle transport, fatty acid metabolism, oxidative phosphorylation, and translational initiation were mainly down-regulated, while those involved in glycolysis, cell cycle arrest, protein catabolism, and cellular amino acid metabolism were up-regulated. The amount of proteins active in the mTOR signaling pathway was generally decreased over time. These results indicate that weaning influences energy metabolism, cellular macromolecule organization and localization, and protein metabolism, thereby affecting the proliferation of intestinal epithelial cells in weaned piglets. Moreover, those cellular processes are possibly controlled by that signaling pathway.
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影响因子：
16
作者：
Sengupta S;Peterson TR;Sabatini DM
通讯作者：
Sabatini DM
影响因子：
4.6
作者：
Stegmann, Anders;Hansen, Morten;Olsen, Jorgen
通讯作者：
Olsen, Jorgen
影响因子：
5.8
作者：
Conesa, A;Götz, S;Robles, M
通讯作者：
Robles, M
DOI：
10.1152/ajpgi.1998.274.1.g131
发表时间：
1998-01-01
影响因子：
4.5
作者：
Dudley, MA;Wykes, LJ;Reeds, PJ
通讯作者：
Reeds, PJ
影响因子：
--
作者：
KATZ, LA;SWAIN, JA;BALABAN, RS
通讯作者：
BALABAN, RS