MicroRNA1917 targets CTR4 splice variants to regulate ethylene responses in tomato
MicroRNA1917 targets CTR4 splice variants to regulate ethylene responses in tomato
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MicroRNA1917靶向CTR4剪接变体来调节番茄中的乙烯反应
DOI：
10.1093/jxb/erx469
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发表时间：
2018-02
影响因子：
6.9
通讯作者：
Li Tianlai
中科院分区：
文献类型：
--
作者：
Wang Yanling;Zou Wenxiong;Xiao Yan;Cheng Lina;Liu Yudong;Gao Song;Shi Zihang;Jiang Yun;Qi Mingfang;Xu Tao;Li Tianlai
乙烯感受受受体调节，下游蛋白质组成性三重反应1是乙烯信号传导的关键抑制因子。非保守的番茄（Solanum lycopersicum）microRNA 1917（Sly-miR 1917）介导SlCTR 4剪接变体（SlCTR 4sv）的降解，但该途径的分子细节仍然未知。Sly-miR 1917和靶向SlCTR 4sv在所有番茄器官中普遍表达。Sly-miR 1917的过表达增强了乙烯反应，包括在没有乙烯的情况下黄化幼苗中的三重反应，以及上位性叶柄生长、加速花梗脱落和果实成熟。在Sly-miR 1917过表达植物（1917-OE）中增强的乙烯信号传导伴随着乙烯生物合成和信号传导基因的上调以及乙烯排放的增加。这些表型通过抑制正乙烯调节因子EIN 2而恢复。此外，在剪接区特异性表达的Sly-miR 1917靶向的SlCTR 4剪接变体SlCTR 4sv 3表现出与Sly-miR 1917相反的表达模式。互补的拟南芥ctr-1突变体和酵母双杂交和双分子荧光互补分析表明，SlCTR 4sv 3功能乙烯信号。Sly-miR 1917和SlCTR 4sv 3在本氏烟草中的共表达进一步表明Sly-miR 1917在体内切割SlCTR 4sv 3。数据库同源性搜索揭示了马铃薯CTR样剪接变体含有Sly-miR 1917结合序列，以及马铃薯中成熟Sly-miR 1917的同源物，表明miR 1917和调节性miRNA介导的乙烯网络在茄科物种中具有保守的功能。
Ethylene perception is regulated by receptors, and the downstream protein CONSTITUTIVE TRIPLE RESPONSE1 is a key suppressor of ethylene signalling. The non-conserved tomato (Solanum lycopersicum) microRNA1917 (Sly-miR1917) mediates degradation of SlCTR4 splice variants (SlCTR4sv) but the molecular details of this pathway remain unknown. Sly-miR1917 and the targeted SlCTR4sv are ubiquitously expressed in all tomato organs. Overexpression of Sly-miR1917 enhances ethylene responses, including the triple response in etiolated seedlings, in the absence of ethylene, as well as epinastic petiole growth, accelerated pedicel abscission, and fruit ripening. Enhanced ethylene signalling in Sly-miR1917-overexpressing plants (1917-OE) is accompanied by up-regulation of ethylene biosynthesis and signalling genes, and increased ethylene emission. These phenotypes were recovered by repressing the positive ethylene regulator EIN2. Moreover, the Sly-miR1917-targeted SlCTR4 splice variant SlCTR4sv3, expressed specifically in the abscission zone, exhibited the opposite expression pattern to Sly-miR1917. Complementation of the Arabidopsis thaliana ctr-1 mutant and yeast two-hybrid and bimolecular fluorescence complementation assays suggested that SlCTR4sv3 functions in ethylene signalling. Co-expression of Sly-miR1917 and SlCTR4sv3 in Nicotiana benthamiana further suggested that Sly-miR1917 cleaves SlCTR4sv3 in vivo. Database homology searching revealed a Solanum tuberosum CTR-like splice variant containing a Sly-miR1917 binding sequence, and a homologue of mature Sly-miR1917 in potato, indicating a conserved function for miR1917 and the regulatory miRNA-mediated ethylene network in solanaceous species.
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影响因子：
6.9
作者：
Agustí J;Merelo P;Cercós M;Tadeo FR;Talón M
通讯作者：
Talón M
影响因子：
4.6
作者：
Wu, Gang;Poethig, R. Scott
通讯作者：
Poethig, R. Scott
DOI：
10.1016/j.plaphy.2004.07.006
发表时间：
2004-09
期刊：
Plant physiology and biochemistry : PPB
影响因子：
--
作者：
S. Kuroda;Yukio Hirose;M. Shiraishi;E. Davies;S. Abe
通讯作者：
S. Kuroda;Yukio Hirose;M. Shiraishi;E. Davies;S. Abe
影响因子：
7
作者：
Begheldo, Maura;Manganaris, George A.;Tonutti, Pietro
通讯作者：
Tonutti, Pietro
影响因子：
4.5
作者：
Megraw, Molly;Baev, Vesselin;Hatzigeorgiou, Artemis G.
通讯作者：
Hatzigeorgiou, Artemis G.