Glycosylation of mucins present in gastric juice: the effect of helicobacter pylori eradication treatment.
Glycosylation of mucins present in gastric juice: the effect of helicobacter pylori eradication treatment.
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DOI：
10.1007/s10238-010-0113-6
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发表时间：
2011-06
影响因子：
4.6
通讯作者：
Stefanska, Ewa
中科院分区：
文献类型：
--
作者：
Radziejewska, Iwona;Borzym-Kluczyk, Malgorzata;Namiot, Zbigniew;Stefanska, Ewa
这表明胃粘蛋白，特别是一些可以作为碳水化合物受体的特定聚糖结构，参与了与幽门螺杆菌粘附素的相互作用。本研究的主要目的是评估胃液糖蛋白在根除治疗前和结束时的糖基化模式。选取13例临床患者的胃液进行分析。凝胶过滤后获得的空隙体积的混合分数进行ELISA测试。为了评估碳水化合物结构的相对量，使用了凝集素和单克隆抗体。在根除处理结束时，观察到被认为是幽门螺杆菌粘附素受体的MUC 1和MUC 5AC粘蛋白水平和碳水化合物结构的变化。我们的结果支持MUC 5AC和MUC 1与一些特定糖结构参与幽门螺杆菌感染机制的观点。
It is suggested that gastric mucins, and in particular some specific glycan structures that can act as carbohydrate receptors, are involved in the interactions with Helicobacter pylori adhesins. The main aim of our study was to evaluate glycosylation pattern of glycoproteins of gastric juice before and at the end of eradication therapy. Gastric juices were taken from 13 clinical patients and subjected to analysis. Pooled fractions of the void volume obtained after gel filtration were subjected to ELISA tests. To assess the relative amounts of carbohydrate structures, lectins and monoclonal antibodies were used. Changes in the level of MUC 1 and MUC 5AC mucins and of carbohydrate structures, which are suggested to be receptors for Helicobacter pylori adhesins, were observed by the end of the eradication treatment. Our results support the idea about the involvement of MUC 5AC and MUC 1 with some specific sugar structures in the mechanism of Helicobacter pylori infection.
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DOI：
10.1111/j.1365-2672.1993.tb02788.x
发表时间：
1993-10-01
期刊：
JOURNAL OF APPLIED BACTERIOLOGY
影响因子：
--
作者：
PUSZTAI, A;GRANT, G;BARDOCZ, S
通讯作者：
BARDOCZ, S
影响因子：
9.8
作者：
Sheu, BS;Odenbreit, S;Wu, JJ
通讯作者：
Wu, JJ
影响因子：
29.4
作者：
Byrd, JC;Yunker, CK;Bresalier, RS
通讯作者：
Bresalier, RS
影响因子：
29.4
作者：
Lindén, S;Nordman, H;Carlstedt, I
通讯作者：
Carlstedt, I
影响因子：
3
作者：
Orczyk-Pawilowicz, M;Florianski, J;Katnik-Prastowska, I
通讯作者：
Katnik-Prastowska, I