Oil from transgenic Camelina sativa as a source of EPA and DHA in feed for European sea bass (Dicentrarchus labrax L.).
Oil from transgenic Camelina sativa as a source of EPA and DHA in feed for European sea bass (Dicentrarchus labrax L.).
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DOI：
10.1016/j.aquaculture.2020.735759
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发表时间：
2021-01-15
期刊：
影响因子：
--
通讯作者：
Tocher DR
中科院分区：
文献类型：
--
作者：
Betancor MB;MacEwan A;Sprague M;Gong X;Montero D;Han L;Napier JA;Norambuena F;Izquierdo M;Tocher DR
水产养殖是增长最快的食品生产部门，不能继续依赖有限的海洋鱼类资源作为饲料中omega-3 （n-3）长链多不饱和脂肪酸（LC-PUFA）、二十碳五烯酸（EPA; 20:5 n-3）和二十二碳六烯酸（DHA; 22:6n-3）的主要来源。本研究进行了为期4个月的饲养试验，旨在研究从转基因亚麻荠中获得的高水平EPA和DHA的新生油在替代欧洲鲈鱼（Dicentrachus labrax）饲料中的鱼油时对生长性能、组织脂肪酸谱和脂质代谢基因表达的影响。每箱50尾幼鱼（初始体重16.7±0.92 g）饲喂3种等脂异蛋白试验饲料，分别为标准饲料(含鱼油和菜籽油的商业混合饲料（CFO）、转基因亚麻荠籽油（TCO）或鱼油和菜籽油的混合饲料（EFO），饲料中EPA和DHA的含量与TCO饲料的n-3 LC-PUFA含量相匹配。饲喂转基因油的鱼的最终重量与饲喂CFO或EFO的鱼的最终重量没有差异。试验初期饲喂TCO的鱼的生长性能略有下降，这与短暂的采食量减少有关，可能是由生亚麻荠油中的硫代葡萄糖苷引起的。与饲喂其他两种饲料的鱼相比，转基因鱼油提高了鱼片的总n-3 PUFA水平，从而改善了鱼片的营养品质，并使鱼肉中的EPA和DHA保持在与饲喂含有鱼油的饲料相同的水平。肝脏和肠道的代谢反应通常相对温和，尽管饮食中TCO和EFO似乎引发了代谢反应，包括β-氧化（cpt1a）和脂肪酸运输（fabp1）的上调，可能反映了更高水平的LC-PUFA。总的来说，本研究表明，一种陆生油料，Camelina sativa，当被改造成含有高水平的EPA和DHA时，可以取代欧洲鲈鱼饲料中的鱼油，而不会对生长或饲料效率产生有害影响，同时还能保持或增加组织n-3 LC-PUFA含量。从转基因亚麻荠中提取的一种含有EPA和DHA的油在鲈鱼中进行了试验。鱼油和转基因鱼油的生长和饲料效率相似。转基因鱼油提高了鱼片中总n-3 PUFA含量。转基因亚麻荠油可有效替代饲料中的鱼油。
Aquaculture, the fastest growing food production sector cannot continue to rely on finite stocks of marine fish as the primary source of the omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA), eicosapentaenoic acid (EPA; 20:5n3) and docosahexaenoic acid (DHA; 22:6n-3), for feeds. A four-month feeding trial was conducted to investigate the impact of a de novo oil, with high levels of EPA and DHA, obtained from transgenic Camelina sativa on growth performance, tissue fatty acid profiles, and expression of lipid metabolism genes when used as a replacement for fish oil in feed for European seabass (Dicentrachus labrax). Triplicate groups of 50 juvenile fish (initial weight 16.7 ± 0.92 g) per tank were fed for 4 months with one of three isolipidic and isoproteic experimental diets consisting of a standard diet containing a commercial blend of fish oil and rapeseed oil (CFO), a diet containing transgenic Camelina oil (TCO), or a blend of fish oil and rapeseed oil with enhanced levels of EPA and DHA (EFO) formulated to match the n-3 LC-PUFA profile of the TCO feed. Final weight of fish fed the GM-derived oil was not different to fish fed either CFO or EFO. Slight lower growth performance of fish fed TCO at the beginning of the trial was related to transient reduced feed intake, possibly caused by glucosinolates in the raw Camelina sativa oil. The GM-derived oil improved the nutritional quality of the fish fillet by enhancing total n-3 PUFA levels compared to the fish fed the other two feeds, and maintained flesh EPA and DHA at the same levels as in fish fed the diets containing fish oil. The metabolic response in liver and intestine was generally relatively mild although diets TCO and EFO seemed to trigger a metabolic response consisting of an up-regulation of both β-oxidation (cpt1a) and fatty acid transport (fabp1), possibly reflecting higher levels of LC-PUFA. Overall, the present study indicated that an oil of terrestrial origin, Camelina sativa, when engineered to contain high levels of EPA and DHA can replace fish oil in feeds for European seabass with no detrimental impact on growth or feed efficiency, while also maintaining or increasing tissue n-3 LC-PUFA contents. An oil containing EPA and DHA from GM-Camelina sativa was tested in seabass. Growth and feed efficiency were similar in fish fed fish oil and the GM-derived oil. The GM-derived oil enhanced total n-3 PUFA level in fish fillet. Oil from GM-Camelina sativa can effectively replace fish oil in feeds for seabass.
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影响因子：
3.7
作者：
Betancor MB;Li K;Sprague M;Bardal T;Sayanova O;Usher S;Han L;Måsøval K;Torrissen O;Napier JA;Tocher DR;Olsen RE
通讯作者：
Olsen RE
来自转基因Camelina sativa的营养增强油有效地取代了鱼油，作为鱼油的来源。
影响因子：
4.6
作者：
Betancor MB;Sprague M;Usher S;Sayanova O;Campbell PJ;Napier JA;Tocher DR
通讯作者：
Tocher DR
影响因子：
2.9
作者：
Bell, JG;Tocher, DR;Sargent, JR
通讯作者：
Sargent, JR
影响因子：
4.5
作者：
Chee, Wei-Ling;Turchini, Giovanni M.;Ng, Wing-Keong
通讯作者：
Ng, Wing-Keong
影响因子：
3.6
作者：
Benitez-Santana, Tibiabin;Juarez-Carrillo, Eduardo;Soledad Izquierdo, Maria
通讯作者：
Soledad Izquierdo, Maria