Inhibitory effects of 2-methacryloyloxyethyl phosphorylcholine polymer on the adherence of bacteria causing upper respiratory tract infection.
Inhibitory effects of 2-methacryloyloxyethyl phosphorylcholine polymer on the adherence of bacteria causing upper respiratory tract infection.
复制标题
DOI：
10.1080/20002297.2020.1808425
复制
发表时间：
2020-08-20
影响因子：
4.5
通讯作者：
Kawabata M
中科院分区：
文献类型：
--
作者：
Iuchi H;Ohori J;Kyutoku T;Ito K;Kawabata M
我们的目的是研究2-甲基丙烯酰氧乙基磷酰胆碱（MPC）聚合物在体外和体内对肺炎链球菌（Spn）和不可分型流感嗜血杆菌（NTHi）粘附的抑制作用。磷酸胆碱（PC）的表达的21个菌株的每个Spn和NTHi进行了评价，使用荧光激活细胞分选;粘附的细菌底特律562细胞和BALB/c小鼠的鼻粘膜进行了测定。MPC聚合物介导的抑制作用与PC-钥孔血蓝蛋白（PC-KLH）介导的抑制作用进行了比较。在体外实验表明，MPC聚合物的预处理显着抑制粘附的Spn和NTHi的浓度剂量依赖性的方式独立的PC表达。PC表达和MPC聚合物介导的抑制作用之间没有观察到相关性。在Spn和NTHi中，PC-KLH介导的抑制作用与PC表达呈显著负相关。通过体内实验获得了相同的结果。MPC聚合物不影响鼻粘膜的组织学。MPC聚合物可有效减少由Spn和NTHi引起的上呼吸道感染的发生，并可用于开发局部治疗，如局部漱口剂和雾化药物。
We aimed to investigate the inhibitory effect of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer on the adherence of Streptococcus pneumoniae (Spn) and nontypeable Haemophilus influenzae (NTHi) in vitro and in vivo. Phosphorylcholine (PC) expression of 21 strains each of Spn and NTHi was evaluated using fluorescence-activated cell sorting; the adherence of bacteria to Detroit 562 cells and to the nasal mucosa of BALB/c mice was determined. MPC polymer-mediated inhibitory effects were compared with PC-keyhole limpet hemocyanin (PC-KLH)-mediated inhibitory effects. In vitro experiments showed that pretreatment with MPC polymer markedly inhibited the adherence of Spn and NTHi in a concentration dose–dependent manner independently of PC expression. No correlation was observed between PC expression and MPC polymer-mediated inhibitory effects. Contrarily, there was a significant negative correlation between PC-KLH-mediated inhibitory effects and PC expression in Spn and NTHi. The same results were obtained via in vivo experiments. The MPC polymer did not affect the histology of the nasal mucosa. MPC polymer might be effective to reduce the occurrence of upper respiratory tract infection caused by Spn and NTHi and could be applied for the development of local treatments, such as topical gargles and nebulizer medications.
登录
查看更多内容
影响因子：
14
作者：
Konno, Tomohiro;Ishihara, Kazuhiko
通讯作者：
Ishihara, Kazuhiko
影响因子：
3.1
作者：
Tong, HH;Blue, LE;DeMaria, TF
通讯作者：
DeMaria, TF
影响因子：
3.1
作者：
Pang, Bing;Winn, Dana;Swords, W. Edward
通讯作者：
Swords, W. Edward
影响因子：
3.1
作者：
BRILES, DE;FORMAN, C;CRAIN, M
通讯作者：
CRAIN, M
影响因子：
1.7
作者：
Iuchi, Hiroyuki;Ohori, Junichiro;Kurono, Yuichi
通讯作者：
Kurono, Yuichi