Global transcriptional dynamics of diapause induction in non-blood-fed and blood-fed Aedes albopictus.
Global transcriptional dynamics of diapause induction in non-blood-fed and blood-fed Aedes albopictus.
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DOI：
10.1371/journal.pntd.0003724
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发表时间：
2015-04
影响因子：
3.8
通讯作者：
Armbruster PA
中科院分区：
文献类型：
--
作者：
Huang X;Poelchau MF;Armbruster PA
白纹伊蚊是一种日益引起公共卫生关注的媒介，因为它在全球范围内迅速扩张，并能够传播登革热病毒、基孔肯雅病毒和各种其他虫媒病毒。传统的病媒控制策略在很大程度上对Ae无效。白纹伊蚊和新的方法是迫切需要的。光周期滞育是温带昆虫的一种重要生态适应。因此，针对光周期滞育或滞育相关的生理过程的分子调控可以提供新的方法来控制媒介的基础。我们研究了全球转录谱的滞育诱导在Ae。通过对生物学复制的文库进行配对末端RNA-Seq来鉴定白纹伊蚊。我们测序的RNA从整个机构的成年女性饲养下滞育诱导和非滞育诱导光周期有或没有血餐。我们构建了一个全面的转录组组装，结合了以前的组装，并代表了超过14，000个注释的双翅目基因模型。将序列读数映射到转录组，确定了2，251个基因响应滞育诱导的短日照光周期的差异表达。在非血液喂养的女性，滞育诱导的潜在调控元件转录上调，包括两个典型的昼夜节律钟基因，永恒和隐花色素1。在吸血雌性中，与能量产生和后代供应相关的代谢途径中的基因在滞育诱导条件下差异表达，包括氧化磷酸化途径和脂质代谢基因。这项研究是第一个利用强大的RNA-Seq技术来阐明任何昆虫滞育诱导的转录基础。我们确定了调节滞育诱导的候选基因和途径，包括一组保守的基因，这些基因在不同的昆虫群体中作为滞育程序的一部分差异表达。这些基因提供了候选人，其滞育相关的功能，可以进一步询问使用功能基因组学方法在Ae。白纹伊蚊和其他昆虫。白纹伊蚊是一种攻击性的人类叮咬者，能够传播登革热病毒、基孔肯雅病毒和至少22种其他导致人类疾病的病毒。在过去的30年里，这种蚊子已经从其原产的亚洲范围传播到除南极洲以外的所有大陆。控制这种蚊子的努力取得了有限的成功。光周期滞育是指昆虫测量日长（光周期）的能力，作为启动发育停滞（休眠）的线索，以便在不利的季节条件（如冬季）中生存。光周期滞育是一个重要的生态适应，使Ae。白纹伊蚊和其他医学上重要的蚊子栖息在温带环境中并在广泛的地理范围内传播。在这里，我们确定的基因表现出的表达水平的变化（上调或下调）与光周期滞育的诱导在AE。白纹伊蚊这些基因中的一些，基于其在其他生物体中已知的生物学功能，涉及调节光周期滞育，并代表基于遗传或化学破坏这种重要适应的新型病媒控制策略的令人兴奋的目标。
Aedes albopictus is a vector of increasing public health concern due to its rapid global range expansion and ability to transmit Dengue virus, Chikungunya virus and a wide range of additional arboviruses. Traditional vector control strategies have been largely ineffective against Ae. albopictus and novel approaches are urgently needed. Photoperiodic diapause is a crucial ecological adaptation in a wide range of temperate insects. Therefore, targeting the molecular regulation of photoperiodic diapause or diapause-associated physiological processes could provide the basis of novel approaches to vector control. We investigated the global transcriptional profiles of diapause induction in Ae. albopictus by performing paired-end RNA-Seq of biologically replicated libraries. We sequenced RNA from whole bodies of adult females reared under diapause-inducing and non-diapause-inducing photoperiods either with or without a blood meal. We constructed a comprehensive transcriptome assembly that incorporated previous assemblies and represents over 14,000 annotated dipteran gene models. Mapping of sequence reads to the transcriptome identified differential expression of 2,251 genes in response to diapause-inducing short-day photoperiods. In non-blood-fed females, potential regulatory elements of diapause induction were transcriptionally up-regulated, including two of the canonical circadian clock genes, timeless and cryptochrome 1. In blood-fed females, genes in metabolic pathways related to energy production and offspring provisioning were differentially expressed under diapause-inducing conditions, including the oxidative phosphorylation pathway and lipid metabolism genes. This study is the first to utilize powerful RNA-Seq technologies to elucidate the transcriptional basis of diapause induction in any insect. We identified candidate genes and pathways regulating diapause induction, including a conserved set of genes that are differentially expressed as part of the diapause program in a diverse group of insects. These genes provide candidates whose diapause-associated function can be further interrogated using functional genomics approaches in Ae. albopictus and other insects. The mosquito, Aedes albopictus, is an aggressive human biter capable of transmitting Dengue virus, Chikungunya virus and at least 22 additional viruses that cause human illness. Over the last 30 years, this mosquito has spread from its native Asian range to all continents except Antarctica. Efforts to control this mosquito have met with limited success. Photoperiodic diapause refers to the ability of insects to measure day length (photoperiod) as a cue for initiating developmental arrest (dormancy) in order to survive unfavorable seasonal conditions such as winter. Photoperiodic diapause is a crucial ecological adaption that enables Ae. albopictus and other medically important mosquitoes to inhabit temperate environments and spread across broad geographic ranges. Here, we identify genes that exhibit changes in expression levels (up-regulation or down-regulation) in association with the induction of photoperiodic diapause in Ae. albopictus. Some of these genes, based on their known biological function in other organisms, are implicated in regulating photoperiodic diapause and represent exciting targets for novel vector control strategies based on genetic or chemical disruption of this important adaptation.
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影响因子：
2.2
作者：
Brackney, Doug E.;Isoe, Jun;Black, W. C., IV;Zamora, Jorge;Foy, Brian D.;Miesfeld, Roger L.;Olson, Ken E.
通讯作者：
Olson, Ken E.
鉴定与棉铃虫大脑中滞育开始相关的基因表达变化
影响因子：
4.4
作者：
Bao B;Xu WH
通讯作者：
Xu WH
影响因子：
7.4
作者：
Boyd, Olga;Weng, Peter;Zou, Sige
通讯作者：
Zou, Sige
影响因子：
9.6
作者：
Bonizzoni M;Gasperi G;Chen X;James AA
通讯作者：
James AA
影响因子：
3.8
作者：
Esquivel CJ;Cassone BJ;Piermarini PM
通讯作者：
Piermarini PM