Neurite outgrowth on nanofiber scaffolds with different orders, structures, and surface properties.
Neurite outgrowth on nanofiber scaffolds with different orders, structures, and surface properties.
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DOI：
10.1021/nn900070z
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发表时间：
2009-05-26
期刊：
影响因子：
17.1
通讯作者：
Xia, Younan
中科院分区：
文献类型：
--
作者：
Xie, Jingwei;MacEwan, Matthew R.;Li, Xiaoran;Sakiyama-Elbert, Shelly E.;Xia, Younan
静电纺纳米纤维可以很容易地组装成各种类型的支架，用于神经组织工程。本研究的目的是研究和了解初级背根神经节（DRG）在具有不同顺序、结构和表面特性的静电纺丝纳米纤维支架上培养的独特神经突生长模式。我们发现，当在随机定向纳米纤维的无纺布垫上培养时，神经突从DRG主体向外呈放射状延伸，没有特定的方向性。相反，当在平行排列的纳米纤维阵列上培养时，神经突优先沿着纤维的长轴延伸。当在排列和随机纳米纤维区域之间的边界播种时，相同的DRG同时表达排列和随机的神经突场，以响应底层的纳米纤维。当在每层纳米纤维沿不同方向排列的双层支架上培养时，发现神经突依赖于两层纤维的密度。这种双轴模式清楚地表明，神经突的生长可以受到支架不同层的纳米纤维的影响，而不仅仅是最顶层。综上所述，这些结果将为设计用于神经再生应用的纳米纤维支架，以及拓扑结构对神经突生长、生长锥引导和轴突再生的影响提供有价值的信息。
Electrospun nanofibers can be readily assembled into various types of scaffolds for applications in neural tissue engineering. The objective of this study is to examine and understand the unique patterns of neurite outgrowth from primary dorsal root ganglia (DRG) cultured on scaffolds of electrospun nanofibers having different orders, structures, and surface properties. We found that the neurites extended radially outward from the DRG main body without specific directionality when cultured on a nonwoven mat of randomly oriented nanofibers. In contrast, the neurites preferentially extended along the long axis of fiber when cultured on a parallel array of aligned nanofibers. When seeded at the border between regions of aligned and random nanofibers, the same DRG simultaneously expressed aligned and random neurite fields in response to the underlying nanofibers. When cultured on a double-layered scaffold where the nanofibers in each layer were aligned along a different direction, the neurites were found to be dependent on the fiber density in both layers. This bi-axial pattern clearly demonstrates that neurite outgrowth can be influenced by nanofibers in different layers of a scaffold, rather than the topmost layer only. Taken together, these results will provide valuable information pertaining to the design of nanofiber scaffolds for neuroregenerative applications, as well as the effects of topology on neurite outgrowth, growth cone guidance, and axonal regeneration.
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