Plant stem-cell organization and differentiation at single-cell resolution.
Plant stem-cell organization and differentiation at single-cell resolution.
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DOI：
10.1073/pnas.2018788117
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发表时间：
2020-12-29
影响因子：
11.1
通讯作者：
Scanlon MJ
中科院分区：
文献类型：
--
作者：
Satterlee JW;Strable J;Scanlon MJ
植物具有在其整个生命周期中生长和产生新器官的非凡能力，这要归功于其分生组织顶端内持久的多能干细胞群体的活动。在这里，我们从玉米微观顶端分生组织(SAM)中分离出单个细胞，并提供了植物茎分生组织的单细胞转录分析。这项研究使得对玉米顶端的发育遗传组织进行了无偏见的分析，并揭示了SAM动态平衡的进化分化和保守特征。单细胞分析的精细分辨率被用来重建茎细胞分化的过程，在此过程中，干细胞在野生型和突变型玉米幼苗的发育过程中获得不同和不同的细胞命运。植物在顶端分生组织(SAM)中维持着多能干细胞的群体，这些干细胞不断地产生新的地上器官。我们使用单细胞RNA测序(scRNA-seq)来实现对玉米茎干细胞生态位及其分化细胞后代转录格局的无偏见表征。位于SAM顶端的干细胞负责维持基因组的完整性，并表现出较低的细胞分裂率，这与它们对生殖系和体细胞命运的贡献一致。令人惊讶的是，我们没有发现规范的干细胞组织中心替代这些细胞的证据。此外，利用轨迹推断追踪伴随细胞分化的基因表达变化，表明KNOTTED1(KN1)的异源表达加速了细胞分化，促进了玉米叶基的发育。这些对茎尖的单细胞转录分析有助于深入了解玉米幼苗中干细胞的功能和细胞命运的获得过程，并为在细胞水平上更好地剖析植物茎形态发生的遗传控制提供了有价值的支架。
Plants possess the remarkable ability to grow and produce new organs throughout their lifespan, owing to the activities of persistent populations of pluripotent stem cells within their meristematic tips. Here we isolated individual cells from the microscopic shoot apical meristem (SAM) of maize and provide single-cell transcriptomic analysis of a plant shoot meristem. This study enabled an unbiased analysis of the developmental genetic organization of the maize shoot apex and uncovered evolutionarily divergent and conserved signatures of SAM homeostasis. The fine-scale resolution of single-cell analysis was used to reconstruct the process of shoot cell differentiation, whereby stem cells acquire diverse and distinct cell fates over developmental time in wild-type and mutant maize seedlings. Plants maintain populations of pluripotent stem cells in shoot apical meristems (SAMs), which continuously produce new aboveground organs. We used single-cell RNA sequencing (scRNA-seq) to achieve an unbiased characterization of the transcriptional landscape of the maize shoot stem-cell niche and its differentiating cellular descendants. Stem cells housed in the SAM tip are engaged in genome integrity maintenance and exhibit a low rate of cell division, consistent with their contributions to germline and somatic cell fates. Surprisingly, we find no evidence for a canonical stem-cell organizing center subtending these cells. In addition, trajectory inference was used to trace the gene expression changes that accompany cell differentiation, revealing that ectopic expression of KNOTTED1 (KN1) accelerates cell differentiation and promotes development of the sheathing maize leaf base. These single-cell transcriptomic analyses of the shoot apex yield insight into the processes of stem-cell function and cell-fate acquisition in the maize seedling and provide a valuable scaffold on which to better dissect the genetic control of plant shoot morphogenesis at the cellular level.
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影响因子：
64.8
作者：
Cao, Junyue;Spielmann, Malte;Shendure, Jay
通讯作者：
Shendure, Jay
影响因子：
7.4
作者：
Engstrom, Eric M.;Andersen, Carl M.;Bowman, John L.
通讯作者：
Bowman, John L.
影响因子：
11.6
作者：
Johnston, Robyn;Wang, Minghui;Scanlon, Michael J.
通讯作者：
Scanlon, Michael J.
影响因子：
7
作者：
Knauer, Steffen;Javelle, Marie;Timmermans, Marja C. P.
通讯作者：
Timmermans, Marja C. P.
影响因子：
4.3
作者：
MCDANIEL, CN;POETHIG, RS
通讯作者：
POETHIG, RS