Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health.
Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health.
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DOI：
10.1016/j.redox.2016.09.009
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发表时间：
2016-12
期刊：
影响因子：
11.4
通讯作者：
Molina, Anthony J. A.
中科院分区：
文献类型：
--
作者：
Tyrrell, Daniel J.;Bharadwaj, Manish S.;Jorgensen, Matthew J.;Register, Thomas C.;Molina, Anthony J. A.
基于血液的生物能量分析策略正在成为系统性线粒体功能的潜在报告者;然而，这些措施在多大程度上反映了其他组织的生物能量能力尚不清楚。这项工作的前提是，高代谢活性的组织，如骨骼肌和心肌，容易受到全身生物能量能力的差异。因此，我们测试了血细胞、单核细胞和血小板的呼吸能力是否与骨骼肌和心肌线粒体的同期呼吸测定评估相关。本研究检查了18只不同年龄和代谢状态的雌性长尾猴/非洲绿色猴（Chlorocebus aethiops sabaeus）。透化骨骼肌单核细胞和血小板最大容量与最大氧化磷酸化能力相关（R=0.75，95%置信区间[CI]：0.38-0.97; R=0.51，95%CI：0.05-0.81;分别），分离的骨骼肌线粒体呼吸控制比（RCR; R=0.70，95%CI：0.35-0.89; R=0.64，95%CI：0.23-0.98;分别）和分离的心肌线粒体RCR（R=0.55，95%CI：0.22-0.86; R=0.58，95%CI：0.22-0.85;分别）。这些结果表明，基于血液的生物能量分析可用于报告肌肉组织的生物能量能力。血细胞呼吸测定法代表了人类研究中基于组织的线粒体功能评估的一种有吸引力的替代方法，其基于这些测量所需的易于获得和最小的参与者负担。非人灵长类动物循环细胞和肌肉组织的呼吸测定法。血细胞概括了骨骼肌纤维生物能量学的关键方面。血细胞重演骨骼肌和心肌线粒体生物能量学。
Blood based bioenergetic profiling strategies are emerging as potential reporters of systemic mitochondrial function; however, the extent to which these measures reflect the bioenergetic capacity of other tissues is not known. The premise of this work is that highly metabolically active tissues, such as skeletal and cardiac muscle, are susceptible to differences in systemic bioenergetic capacity. Therefore, we tested whether the respiratory capacity of blood cells, monocytes and platelets, are related to contemporaneous respirometric assessments of skeletal and cardiac muscle mitochondria. 18 female vervet/African green monkeys (Chlorocebus aethiops sabaeus) of varying age and metabolic status were examined for this study. Monocyte and platelet maximal capacity correlated with maximal oxidative phosphorylation capacity of permeabilized skeletal muscle (R=0.75, 95% confidence interval [CI]: 0.38–0.97; R=0.51, 95%CI: 0.05–0.81; respectively), isolated skeletal muscle mitochondrial respiratory control ratio (RCR; R=0.70, 95%CI: 0.35–0.89; R=0.64, 95%CI: 0.23–0.98; respectively), and isolated cardiac muscle mitochondrial RCR (R=0.55, 95%CI: 0.22–0.86; R=0.58, 95%CI: 0.22–0.85; respectively). These results suggest that blood based bioenergetic profiling may be used to report on the bioenergetic capacity of muscle tissues. Blood cell respirometry represents an attractive alternative to tissue based assessments of mitochondrial function in human studies based on ease of access and the minimal participant burden required by these measures. Respirometry of circulating cells and muscle tissues from non-human primates. Blood cells recapitulate key aspects of skeletal muscle fiber bioenergetics. Blood cells recapitulate skeletal and cardiac muscle mitochondrial bioenergetics.
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影响因子：
4.4
作者：
Fincham, JE;Benade, AJS;Kritchevsky, D
通讯作者：
Kritchevsky, D
DOI：
10.1007/978-1-59745-521-3_17
发表时间：
2009-01-01
期刊：
MITOCHONDRIAL DNA: METHODS AND PROTOCOLS
影响因子：
--
作者：
Griffiths, Lyra M.;Doudican, Nicole A.;Doetsch, Paul W.
通讯作者：
Doetsch, Paul W.
影响因子：
16.2
作者：
Bonora, E;Saggiani, F;Muggeo, M
通讯作者：
Muggeo, M
影响因子：
4.7
作者：
Desler C;Hansen TL;Frederiksen JB;Marcker ML;Singh KK;Juel Rasmussen L
通讯作者：
Juel Rasmussen L
影响因子：
3.5
作者：
Hartman, Mor-Li;Shirihai, Orian S.;Vita, Joseph A.
通讯作者：
Vita, Joseph A.