Leptospira spp in Rodents from Peridomestic Sites in Endemic Regions of Nicaragua.
Leptospira spp in Rodents from Peridomestic Sites in Endemic Regions of Nicaragua.
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DOI：
10.1007/s10393-021-01520-4
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发表时间：
2020-12
期刊：
影响因子：
2.5
通讯作者：
Duttmann, Christiane
中科院分区：
文献类型：
--
作者：
Flores, Byron;Sheleby-Elias, Jessica;Perez-Sanchez, Tania;Fischer, Rebecca;Muzquiz, Jose;Fuertes, Hector;Halaihel, Nabil;Jiron, William;Duttmann, Christiane
钩端螺旋体病是热带国家最重要的人畜共患病之一，其中尼加拉瓜被认为是地方病。本研究的目的是确定从尼加拉瓜钩端螺旋体病流行区的家周围捕获的啮齿动物中钩端螺旋体属的频率。 2012 年和 2013 年 4 月至 12 月期间，使用活体诱捕器捕获了 191 只啮齿动物。通过直接培养和分离，收集并处理 166 只动物的肾脏样本，用于钩端螺旋体检测。通过 PCR 检测分离株中致病性钩端螺旋体特异性的 LipL32 和 lfb1-F 基因。所有地点的诱捕成功率为 20.2%，雨季的成功率更高 (p < 0.05)。通过直接培养方法在 22.3% 的啮齿动物中检测到钩端螺旋体。每月以及每个地区钩端螺旋体阳性啮齿动物的频率存在显着差异（p < 0.01）。在分离的钩端螺旋体中，PCR 检测致病菌阳性率为 37.5%。分离的钩端螺旋体阳性啮齿动物的频率可用作尼加拉瓜人类钩端螺旋体病风险的预测指标。
Leptospirosis is one of the most important zoonoses in tropical countries, including Nicaragua, where it is considered endemic. The aim of this study was to determine the frequency of Leptospira spp in rodents captured from peridomestic sites in leptospirosis endemic regions of Nicaragua. Using live traps, 191 rodents were captured in 2012 and 2013 between April and December. Kidney samples were collected and processed for Leptospira detection from 166 animals by direct culture and isolation. The isolates were tested by PCR for LipL32 and lfb1-F genes specific to pathogenic Leptospira species. The trapping success over all sites was 20.2%, with higher rates of success in rainy season (p < 0.05). Leptospira spp were detected in 22.3% of rodents by direct culture methods. Significant differences (p < 0.01) were found in the frequencies of Leptospira positive rodents per month as well as per region. Of the isolated Leptospira spp, 37.5% were positive for pathogenic species by PCR. The frequency of Leptospira positive rodents by isolation could be used as a predictive indicator for the risk of human leptospirosis in Nicaragua.
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影响因子：
2.7
作者：
Flores, Byron J.;Perez-Sanchez, Tania;Halaihel, Nabil
通讯作者：
Halaihel, Nabil
影响因子：
3.1
作者：
Haake, DA;Chao, G;Bolin, CA
通讯作者：
Bolin, CA
DOI：
10.3390/ijerph110606000
发表时间：
2014-06-05
影响因子：
--
作者：
Durski KN;Jancloes M;Chowdhary T;Bertherat E
通讯作者：
Bertherat E
DOI：
10.3390/ijerph110808301
发表时间：
2014-08-01
影响因子：
--
作者：
Bacallao, Jorge;Schneider, Maria Cristina;Espinal, Marcos A.
通讯作者：
Espinal, Marcos A.
影响因子：
3.3
作者：
Halliday, Jo E. B.;Knobel, Darryn L.;Breiman, Robert F.
通讯作者：
Breiman, Robert F.