In vivo administration of recombinant growth hormone or gamma interferon activities macrophages: enhanced resistance to experimental Salmonella typhimurium infection is correlated with generation of reactive oxygen intermediates
In vivo administration of recombinant growth hormone or gamma interferon activities macrophages: enhanced resistance to experimental Salmonella typhimurium infection is correlated with generation of reactive oxygen intermediates
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体内施用重组生长激素或γ干扰素活性巨噬细胞：对实验性鼠伤寒沙门氏菌感染的抵抗力增强与活性氧中间体的产生相关
作者：
C. Edwards;S. M. Ghiasuddin;L. Yunger;R. Lorence;S. Arkins;R. Dantzer;K. Kelley
纯化和重组形式的生长激素（GH），以及重组大鼠γ干扰素（IFN-γ）增强大鼠的生存剥夺内源性垂体GH分泌垂体切除术（HX大鼠）和感染毒鼠伤寒沙门氏菌。与安慰剂治疗的动物相比，在体外调理酵母聚糖刺激后，从垂体完整的大鼠（垂体完整的大鼠）或HX大鼠中获得的巨噬细胞释放的超氧阴离子（O2-）水平升高（P <0.05），在体内用GH或密切相关的激素催乳素治疗。这些水平的O2-释放的幅度相似的巨噬细胞在体内用IFN-γ治疗。在体内巨噬细胞活化研究的时间过程中，IFN-γ和GH均在24小时内显著增加O2-分泌，最大分泌发生在第3天。从垂体完整和HX大鼠体内注射GH获得的巨噬细胞也释放升高（P <0.05）的过氧化氢（H2 O2）水平，并显示增强（P <0.01）的吞噬活性对调理单核细胞增生李斯特菌在体外。GH在体内的作用机制很可能是直接的，因为大鼠的常驻腹腔巨噬细胞可以在体外引发这些细胞与调理酵母多糖触发后增强O2-的分泌。这些数据表明，在体内给予两种密切相关的垂体激素，GH和催乳素，可以有效地启动巨噬细胞，这与GH介导对S.通过对巨噬细胞的直接刺激作用，
Purified and recombinant forms of growth hormone (GH) as well as of recombinant rat gamma interferon (IFN-gamma) enhance the survival of rats deprived of endogenous pituitary GH secretion by hypophysectomy (HX rats) and infected with virulent Salmonella typhimurium. Macrophages obtained from rats with intact pituitaries (pituitary-intact rats) or HX rats that were treated in vivo with either GH or the closely related hormone prolactin released elevated (P less than 0.05) levels of superoxide anion (O2-) after in vitro opsonized-zymosan stimulation compared with those from placebo-treated animals. These levels of O2- release were similar in magnitude to those of macrophages from rats treated in vivo with IFN-gamma. In time course in vivo macrophage activation studies, both IFN-gamma and GH significantly increased O2- secretion within 24 h, with maximal secretion occurring at day 3. Macrophages obtained from pituitary-intact and HX rats injected in vivo with GH also released elevated (P less than 0.05) levels of hydrogen peroxide (H2O2) and displayed enhanced (P less than 0.01) phagocytic activity toward opsonized Listeria monocytogenes in vitro. The mechanism of action of GH in vivo is likely to be a direct one because resident peritoneal macrophages from rats could be primed in vitro for enhanced secretion of O2- following triggering of these cells with opsonized zymosan. These data show that in vivo administration of two closely related pituitary hormones, GH and prolactin, can effectively prime macrophages, which is consistent with the hypothesis that GH mediates resistance to S. typhimurium by a direct stimulatory action on macrophages.
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影响因子：
5.8
作者：
K. W. Kelley
通讯作者：
K. W. Kelley
巨噬细胞分泌有毒氧产物：调节细胞因子及其对氧化酶的影响。
DOI：
10.1002/9780470720998.ch14
发表时间：
1986
期刊：
Ciba Foundation symposium
影响因子：
--
作者：
Nathan,CF;Tsunawaki,S
通讯作者：
Tsunawaki,S
小鼠细胞内分枝杆菌引起的慢性感染：与巨噬细胞释放前列腺素 E2 以及注射吲哚美辛、胞壁酰二肽或干扰素-γ 逆转相关。
DOI：
--
发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Edwards3rd,CK;Hedegaard,HB;Zlotnik,A;Gangadharam,PR;JohnstonJr,RB;Pabst,MJ
通讯作者：
Pabst,MJ
生长激素通过与催乳素受体结合来增强人中性粒细胞的超氧阴离子分泌。
DOI：
10.1172/jci115605
发表时间：
1992
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Fu,YK;Arkins,S;Fuh,G;Cunningham,BC;Wells,JA;Fong,S;Cronin,MJ;Dantzer,R;Kelley,KW
通讯作者：
Kelley,KW
DOI：
10.1073/pnas.83.14.5189
发表时间：
1986-07-01
影响因子：
11.1
作者：
FIELDS, PI;SWANSON, RV;HEFFRON, F
通讯作者：
HEFFRON, F