Effective and specific in planta RNAi in cyst nematodes: expression interference of four parasitism genes reduces parasitic success.
Effective and specific in planta RNAi in cyst nematodes: expression interference of four parasitism genes reduces parasitic success.
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DOI：
10.1093/jxb/ern289
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发表时间：
2009
影响因子：
6.9
通讯作者：
Baum TJ
中科院分区：
文献类型：
--
作者：
Sindhu AS;Maier TR;Mitchum MG;Hussey RS;Davis EL;Baum TJ
孢囊线虫是一种高度进化的定居植物内寄生虫，它利用通过口针注射到宿主组织中的寄生蛋白来成功地寄生植物。这些分泌蛋白可能是寄生所必需的，因为它们参与了各种寄生事件，导致线虫获得营养所需的专门的摄食细胞的建立。随着RNA干扰（RNAi）技术的出现和寄生虫中宿主诱导的基因沉默的展示，防治害虫和病原体的新策略已经可用，特别是在根结线虫中。到目前为止，植物宿主诱导的胞囊线虫基因沉默只取得了有限的成功，但同样应该破坏寄生循环，使宿主植物产生抗性。通过在转基因拟南芥中通过宿主诱导的RNAi基因沉默靶向四个寄生基因，提供了用于胞囊线虫的额外的植物内RNAi数据，所述转基因拟南芥是甜菜胞囊线虫Heterodera schachtii的宿主。本文报道了在以表达相应RNAi构建体的植物为食的线虫中，靶向线虫基因的mRNA丰度特异性降低。此外，所有四种线虫寄生基因的这种宿主诱导的RNAi导致成熟线虫雌性数量的减少。虽然没有观察到完全的抗性，但在不同的RNAi品系中，发育中的雌性的减少范围从23%至64%。这些观察结果证明了线虫生命周期中靶向寄生基因的相关性，并且可能更重要的是，表明未来可能使用这种技术对抗胞囊线虫来实现作物植物中可行的抗性水平。
Cyst nematodes are highly evolved sedentary plant endoparasites that use parasitism proteins injected through the stylet into host tissues to successfully parasitize plants. These secretory proteins likely are essential for parasitism as they are involved in a variety of parasitic events leading to the establishment of specialized feeding cells required by the nematode to obtain nourishment. With the advent of RNA interference (RNAi) technology and the demonstration of host-induced gene silencing in parasites, a new strategy to control pests and pathogens has become available, particularly in root-knot nematodes. Plant host-induced silencing of cyst nematode genes so far has had only limited success but similarly should disrupt the parasitic cycle and render the host plant resistant. Additional in planta RNAi data for cyst nematodes are being provided by targeting four parasitism genes through host-induced RNAi gene silencing in transgenic Arabidopsis thaliana, which is a host for the sugar beet cyst nematode Heterodera schachtii. Here it is reported that mRNA abundances of targeted nematode genes were specifically reduced in nematodes feeding on plants expressing corresponding RNAi constructs. Furthermore, this host-induced RNAi of all four nematode parasitism genes led to a reduction in the number of mature nematode females. Although no complete resistance was observed, the reduction of developing females ranged from 23% to 64% in different RNAi lines. These observations demonstrate the relevance of the targeted parasitism genes during the nematode life cycle and, potentially more importantly, suggest that a viable level of resistance in crop plants may be accomplished in the future using this technology against cyst nematodes.
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影响因子：
17.3
作者：
Gheysen, Godelieve;Vanholme, Bartel
通讯作者：
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影响因子：
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影响因子：
7.2
作者：
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