Identification of Potential Biomarkers and Related Transcription Factors in Peripheral Blood of Tuberculosis Patients.
Identification of Potential Biomarkers and Related Transcription Factors in Peripheral Blood of Tuberculosis Patients.
复制标题
结核病患者外周血中潜在生物标志物及相关转录因子的鉴定
DOI：
10.3390/ijerph17196993
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发表时间：
2020-09-24
影响因子：
--
通讯作者：
Xie J
中科院分区：
文献类型：
--
作者：
Xie L;Chao X;Teng T;Li Q;Xie J
Tuberculosis (TB), one major threat to humans, can infect one third of the worldwide population, and cause more than one million deaths each year. This study aimed to identify the effective diagnosis and therapy biomarkers of TB. Hence, we analyzed two microarray datasets (GSE54992 and GSE62525) derived from the Gene Expression Omnibus (GEO) database to find the differentially expressed genes (DEGs) of peripheral blood mononuclear cell (PBMC) between TB patients and healthy specimens. Functional and pathway enrichment of the DEGs were analyzed by Metascape database. Protein-protein interaction (PPI) network among the DEGs were constructed by STRING databases and visualized in Cytoscape software. The related transcription factors regulatory network of the DEGs was also constructed. A total of 190 DEGs including 36 up-regulated genes and 154 down-regulated genes were obtained in TB samples. Gene functional enrichment analysis showed that these DEGs were enriched in T cell activation, chemotaxis, leukocyte activation involved in immune response, cytokine secretion, head development, etc. The top six hub genes (namely, LRRK2, FYN, GART, CCR7, CXCR5, and FASLG) and two significant modules were got from PPI network of DEGs. Vital transcriptional factors, such as FoxC1 and GATA2, were discovered with close interaction with these six hub DEGs. By systemic bioinformatic analysis, many DEGs associated with TB were screened, and these identified hub DEGs may be potential biomarkers for diagnosis and treatment of TB in the future.
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影响因子：
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作者：
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通讯作者：
Ferreira, Antonio Walter
影响因子：
17.1
作者：
Singhal, Amit;Jie, Liu;De Libero, Gennaro
通讯作者：
De Libero, Gennaro
影响因子：
3.6
作者：
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通讯作者：
Bahar, Bojlul
补体 C1q 水平升高标志着人类结核病处于活动状态。
影响因子：
3.7
作者：
Cai Y;Yang Q;Tang Y;Zhang M;Liu H;Zhang G;Deng Q;Huang J;Gao Z;Zhou B;Feng CG;Chen X
通讯作者：
Chen X
影响因子：
8
作者：
Roe JK;Thomas N;Gil E;Best K;Tsaliki E;Morris-Jones S;Stafford S;Simpson N;Witt KD;Chain B;Miller RF;Martineau A;Noursadeghi M
通讯作者：
Noursadeghi M