Polyvinyl chloride degradation by a bacterium isolated from the gut of insect larvae.
Polyvinyl chloride degradation by a bacterium isolated from the gut of insect larvae.
复制标题
从昆虫幼虫肠道中分离出的细菌降解聚氯乙烯
DOI：
10.1038/s41467-022-32903-y
复制
发表时间：
2022-09-12
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
微生物降解聚氯乙烯（PVC）的证据以前已经报道，但很少有人知道的降解菌株和酶。在这里，我们从昆虫幼虫的肠道中分离出一种PVC降解细菌，并使用多组学方法阐明PVC降解途径。我们发现，昆虫害虫，草地贪夜蛾，幼虫可以生存的PVC薄膜喂养，这是与丰富的肠球菌，克雷伯氏菌和其他细菌在幼虫的肠道微生物群。从幼虫肠道分离的一株细菌（克雷伯氏菌EMBL-1）能够降解和利用PVC作为唯一的能源。我们使用基因组学、转录组学、蛋白质组学和代谢组学分析来鉴定可能参与PVC降解的基因和蛋白质（例如，过氧化氢酶-过氧化物酶、脱卤酶、烯醇酶、醛脱氢酶和加氧酶），并提出了PVC生物降解途径。此外，使用纯化的过氧化氢酶-过氧化物酶的酶测定支持在PVC解聚中的作用。聚氯乙烯（PVC）的微生物降解已有报道，但对降解菌和降解酶的了解甚少。在这里，Zhe等人从昆虫幼虫的肠道中分离出一种PVC降解细菌，并使用多组学方法阐明了PVC降解途径。
Evidence for microbial degradation of polyvinyl chloride (PVC) has previously been reported, but little is known about the degrading strains and enzymes. Here, we isolate a PVC-degrading bacterium from the gut of insect larvae and shed light on the PVC degradation pathway using a multi-omic approach. We show that the larvae of an insect pest, Spodoptera frugiperda, can survive by feeding on PVC film, and this is associated with enrichment of Enterococcus, Klebsiella and other bacteria in the larva’s gut microbiota. A bacterial strain isolated from the larval intestine (Klebsiella sp. EMBL-1) is able to depolymerize and utilize PVC as sole energy source. We use genomic, transcriptomic, proteomic, and metabolomic analyses to identify genes and proteins potentially involved in PVC degradation (e.g., catalase-peroxidase, dehalogenases, enolase, aldehyde dehydrogenase and oxygenase), and propose a PVC biodegradation pathway. Furthermore, enzymatic assays using the purified catalase-peroxidase support a role in PVC depolymerization. Microbial degradation of polyvinyl chloride (PVC) has previously been reported, but little is known about the degrading strains and enzymes. Here, Zhe et al. isolate a PVC-degrading bacterium from the gut of insect larvae and shed light on the PVC degradation pathway using a multi-omic approach.
登录
查看更多内容
影响因子：
5.2
作者：
Deangelis KM;Sharma D;Varney R;Simmons B;Isern NG;Markilllie LM;Nicora C;Norbeck AD;Taylor RC;Aldrich JT;Robinson EW
通讯作者：
Robinson EW
影响因子：
11.4
作者：
Gravouil, Kevin;Ferru-Clement, Romain;Ferreira, Thierry
通讯作者：
Ferreira, Thierry
影响因子：
3.3
作者：
Giacomucci, Lucia;Raddadi, Noura;Fava, Fabio
通讯作者：
Fava, Fabio
影响因子：
5.3
作者：
Fujisawa, M;Hirai, H;Nishida, T
通讯作者：
Nishida, T
DOI：
10.1016/j.ibiod.2016.06.012
发表时间：
2016-10-01
影响因子：
4.8
作者：
Jeon, Hyun Jeong;Kim, Mal Nam
通讯作者：
Kim, Mal Nam