Single-Cell Transcriptomics Reveals the Molecular Anatomy of Sheep Hair Follicle Heterogeneity and Wool Curvature.
Single-Cell Transcriptomics Reveals the Molecular Anatomy of Sheep Hair Follicle Heterogeneity and Wool Curvature.
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单细胞转录组学揭示了绵羊毛囊异质性和羊毛曲率的分子解剖学
DOI：
10.3389/fcell.2021.800157
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发表时间：
2021
影响因子：
5.5
通讯作者：
Sun W
中科院分区：
文献类型：
--
作者：
Wang S;Wu T;Sun J;Li Y;Yuan Z;Sun W
羊毛是重要的纺织原料，它是由绵羊的毛囊生产的。因此，研究毛囊发育的分子机制具有重要意义。由于绵羊毛囊高度的细胞异质性以及不充分的细胞、分子和空间特征，绵羊毛囊发育和羊毛弯曲的分子机制仍不清楚。单细胞RNA测序（scRNA-seq）技术使全面剖析复杂皮肤组织的细胞组成成为可能，并揭示了表皮和毛囊发育的分化和空间特征。然而，迄今为止，这种研究在羊身上还很缺乏。在此，制备来自卷曲羊毛和直羊毛毛皮的单细胞悬浮液用于无偏scRNA-seq。基于UAMP降维分析，我们从15，830个单细胞转录组中鉴定了19个不同的细胞群，并根据特定的基因表达谱表征了它们的细胞身份。此外，新的标记基因被应用于毛乳头细胞的鉴定。利用伪时间序列分析方法，构建了毛干细胞分化轨迹，揭示了毛干细胞和内根鞘（IRS）细胞定向分化的动态基因表达谱。同时，间充质和上皮细胞之间的细胞间通讯推断CellChat和配体受体对的先验知识的基础上。结果，揭示了强的细胞间通讯和相关的信号通路。此外，为了阐明羊毛弯曲的分子机制，对直毛和卷曲毛特异细胞中差异表达的基因进行了鉴定和分析。我们的研究结果提供了一个公正和系统的绵羊毛囊的分子解剖学包括19簇;揭示了分化，空间特征，和细胞间通讯的绵羊毛囊发育的基础，并在同一时间揭示了羊毛弯曲的潜在分子机制，这将提供重要的新见解，绵羊毛囊的生物学和绵羊育种的影响。
Wool is the critical textile raw material which is produced by the hair follicle of sheep. Therefore, it has important implications to investigate the molecular mechanism governing hair follicle development. Due to high cellular heterogeneity as well as the insufficient cellular, molecular, and spatial characterization of hair follicles on sheep, the molecular mechanisms involved in hair follicle development and wool curvature of sheep remains largely unknown. Single-cell RNA sequencing (scRNA-seq) technologies have made it possible to comprehensively dissect the cellular composition of complex skin tissues and unveil the differentiation and spatial signatures of epidermal and hair follicle development. However, such studies are lacking so far in sheep. Here, single-cell suspensions from the curly wool and straight wool lambskins were prepared for unbiased scRNA-seq. Based on UAMP dimension reduction analysis, we identified 19 distinct cell populations from 15,830 single-cell transcriptomes and characterized their cellular identity according to specific gene expression profiles. Furthermore, novel marker gene was applied in identifying dermal papilla cells isolated in vitro. By using pseudotime ordering analysis, we constructed the matrix cell lineage differentiation trajectory and revealed the dynamic gene expression profiles of matrix progenitors' commitment to the hair shaft and inner root sheath (IRS) cells. Meanwhile, intercellular communication between mesenchymal and epithelial cells was inferred based on CellChat and the prior knowledge of ligand–receptor pairs. As a result, strong intercellular communication and associated signaling pathways were revealed. Besides, to clarify the molecular mechanism of wool curvature, differentially expressed genes in specific cells between straight wool and curly wool were identified and analyzed. Our findings here provided an unbiased and systematic view of the molecular anatomy of sheep hair follicle comprising 19 clusters; revealed the differentiation, spatial signatures, and intercellular communication underlying sheep hair follicle development; and at the same time revealed the potential molecular mechanism of wool curvature, which will give important new insights into the biology of the sheep hair follicle and has implications for sheep breeding.
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DOI：
10.1016/j.alit.2014.06.001
发表时间：
2015-01
期刊：
Allergology international : official journal of the Japanese Society of Allergology
影响因子：
--
作者：
Arima K;Ohta S;Takagi A;Shiraishi H;Masuoka M;Ontsuka K;Suto H;Suzuki S;Yamamoto K;Ogawa M;Simmons O;Yamaguchi Y;Toda S;Aihara M;Conway SJ;Ikeda S;Izuhara K
通讯作者：
Izuhara K
影响因子：
3.6
作者：
Chovatiya G;Ghuwalewala S;Walter LD;Cosgrove BD;Tumbar T
通讯作者：
Tumbar T
影响因子：
3.8
作者：
Choi, Jee-Hyun;Kim, Byong-Kyu;Yoon, Sungjoo Kim
通讯作者：
Yoon, Sungjoo Kim
单细胞转录组分析揭示胚胎毛囊发育过程中真皮和上皮细胞的命运决定
影响因子：
12.4
作者：
Ge, Wei;Tan, Shao-Jing;Wang, Xin
通讯作者：
Wang, Xin
影响因子：
6.5
作者：
Batch, JA;Mercuri, FA;Werther, GA
通讯作者：
Werther, GA