Let it go: mechanisms that detach myosin V from the yeast vacuole.
Let it go: mechanisms that detach myosin V from the yeast vacuole.
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DOI：
10.1007/s00294-021-01195-y
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发表时间：
2021-12
期刊：
影响因子：
2.5
通讯作者：
Weisman LS
中科院分区：
文献类型：
--
作者：
Wong S;Weisman LS
细胞生物学中的一个主要问题是，细胞器和大分子机器如何在细胞内移动？在细胞内正确的时间将货物运送到正确的地方对细胞健康至关重要。如果不这样做，对动物生理学来说往往是灾难性的，并导致肠道、大脑和皮肤疾病。在芽殖酵母中，肌球蛋白V马达Myo2将细胞物质从母细胞移动到生长中的子芽中。基于Myo2的运输确保细胞内容物在细胞分裂期间共享。在运输过程中，Myo2通常通过货物特异性衔接蛋白与其货物连接。因此，这种简单的生物体是研究肌球蛋白V如何移动货物（如细胞器）的有力工具。一些关键的问题包括肌球蛋白V如何沿着肌动蛋白细胞骨架移动，或者肌球蛋白V如何附着在母体中的货物上。其他关键问题包括当肌球蛋白V到达其最终目的地时，货物是如何从肌球蛋白V中释放出来的。在这里，我们回顾了调节液泡特异性衔接蛋白Vac17的机制，以确保Myo2将液泡传递到芽中，并在正确的位置和正确的时间释放它。最近的研究表明，Vac17是由泛素化和磷酸化事件，协调其降解和从Myo2的液泡的脱离调节。因此，多种翻译后修饰紧密协调货物递送与细胞事件。人们很容易推测，类似的机制也调节着其他货物和分子马达。
A major question in cell biology is, how are organelles and macromolecular machines moved within a cell? The delivery of cargoes to the right place at the right time within a cell is critical to cellular health. Failure to do so is often catastrophic for animal physiology and results in diseases of the gut, brain, and skin. In budding yeast, a myosin V motor, Myo2, moves cellular materials from the mother cell into the growing daughter bud. Myo2-based transport ensures that cellular contents are shared during cell division. During transport, Myo2 is often linked to its cargo via cargo-specific adaptor proteins. This simple organism thus serves as a powerful tool to study how myosin V moves cargo, such as organelles. Some critical questions include how myosin V moves along the actin cytoskeleton, or how myosin V attaches to cargo in the mother. Other critical questions include how the cargo is released from myosin V when it reaches its final destination in the bud. Here, we review the mechanisms that regulate the vacuole specific adaptor protein, Vac17, to ensure that Myo2 delivers the vacuole to the bud and releases it at the right place and the right time. Recent studies have revealed that Vac17 is regulated by ubiquitylation and phosphorylation events that coordinate its degradation and the detachment of the vacuole from Myo2. Thus, multiple post-translational modifications tightly coordinate cargo delivery with cellular events. It is tempting to speculate that similar mechanisms regulate other cargoes and molecular motors.
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DOI：
10.1083/jcb.128.5.779
发表时间：
1995-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Vida TA;Emr SD
通讯作者：
Emr SD
DOI：
10.1083/jcb.201201024
发表时间：
2012-07-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
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通讯作者：
Weisman LS
影响因子：
11.8
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通讯作者：
Weisman, Lois S.
影响因子：
4
作者：
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通讯作者：
Rachubinski, Richard A.
影响因子：
4.5
作者：
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通讯作者：
Weisman, Lois S.