Somatic CG6015 mediates cyst stem cell maintenance and germline stem cell differentiation via EGFR signaling in Drosophila testes.
Somatic CG6015 mediates cyst stem cell maintenance and germline stem cell differentiation via EGFR signaling in Drosophila testes.
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体细胞CG6015通过果蝇睾丸中的EGFR信号传导介导囊肿干细胞维持和生殖干细胞分化
DOI：
10.1038/s41420-021-00452-w
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发表时间：
2021-04-06
影响因子：
7
通讯作者：
Yu J
中科院分区：
文献类型：
--
作者：
Zheng Q;Chen X;Qiao C;Wang M;Chen W;Luan X;Yan Y;Shen C;Fang J;Hu X;Zheng B;Wu Y;Yu J
干细胞生态位受内在和外在因素的调控。在果蝇睾丸中，囊肿干细胞（CySCs）支持生殖系干细胞（GSCs）的分化。然而，潜在的机制仍不清楚。在这项研究中，我们发现在果蝇模型中，体细胞CG6015是维持GSC和GSC分化所必需的。在CySCs中敲低CG6015导致果蝇睾丸未分化生殖细胞中dpERK的异常激活，并且破坏CySCs中EGFR信号通路的关键下游靶点（Dsor1和rl）导致与CG6015敲低相似的表型。CG6015、Dsor1和rl对果蝇Schneider 2 （S2）细胞的存活至关重要。我们的数据表明，体细胞CG6015通过EGFR信号调节CySC维持和GSC分化，并抑制胚系dpERK信号的异常激活。这些发现提示了睾丸果蝇干细胞生态位稳态的调控机制。
Stem cell niche is regulated by intrinsic and extrinsic factors. In the Drosophila testis, cyst stem cells (CySCs) support the differentiation of germline stem cells (GSCs). However, the underlying mechanisms remain unclear. In this study, we found that somatic CG6015 is required for CySC maintenance and GSC differentiation in a Drosophila model. Knockdown of CG6015 in CySCs caused aberrant activation of dpERK in undifferentiated germ cells in the Drosophila testis, and disruption of key downstream targets of EGFR signaling (Dsor1 and rl) in CySCs results in a phenotype resembling that of CG6015 knockdown. CG6015, Dsor1, and rl are essential for the survival of Drosophila cell line Schneider 2 (S2) cells. Our data showed that somatic CG6015 regulates CySC maintenance and GSC differentiation via EGFR signaling, and inhibits aberrant activation of germline dpERK signals. These findings indicate regulatory mechanisms of stem cell niche homeostasis in the Drosophila testis.
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影响因子：
8.8
作者：
Filippi S;Barnes CP;Kirk PD;Kudo T;Kunida K;McMahon SS;Tsuchiya T;Wada T;Kuroda S;Stumpf MP
通讯作者：
Stumpf MP
影响因子：
4.6
作者：
Cheng, Jun;Tiyaboonchai, Amita;Hunt, Alan J.
通讯作者：
Hunt, Alan J.
影响因子：
2.5
作者：
Kerins, Jessica Amrozowicz;Hanazawa, Momoyo;Dorsett, Maia;Schedl, Tim
通讯作者：
Schedl, Tim
影响因子：
4
作者：
Hall, Eric T.;Verheyen, Esther M.
通讯作者：
Verheyen, Esther M.
DOI：
10.1007/978-1-4939-7219-7_2
发表时间：
2017-01-01
期刊：
ERBB RECEPTOR SIGNALING: METHODS AND PROTOCOLS
影响因子：
--
作者：
Harden, Nicholas
通讯作者：
Harden, Nicholas