PRP-17 and the pre-mRNA splicing pathway are preferentially required for the proliferation versus meiotic development decision and germline sex determination in Caenorhabditis elegans.
PRP-17 and the pre-mRNA splicing pathway are preferentially required for the proliferation versus meiotic development decision and germline sex determination in Caenorhabditis elegans.
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DOI：
10.1002/dvdy.22274
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发表时间：
2010-05
影响因子：
2.5
通讯作者：
Schedl, Tim
中科院分区：
文献类型：
--
作者：
Kerins, Jessica Amrozowicz;Hanazawa, Momoyo;Dorsett, Maia;Schedl, Tim
关键词：
在线虫中，生殖系干细胞的增殖和进入减数分裂的决定是由GLP-1 Notch信号控制的，GLP-1 Notch信号通过抑制指导减数分裂进入的冗余GLD-1和GLD-2途径来促进增殖。我们确认PRP-17是GLP-1信号下游的另一个基因，它主要通过作用于GLD-1途径促进减数分裂进入，也在雌性生殖系性别决定中发挥作用。PRP-17与酵母和人前mRNA剪接因子PRP17/CDC40同源，可以挽救酵母PRP17对温度敏感的致死性。这种与剪接的联系导致了在致敏遗传背景中预测线虫剪接因子的RNAi屏幕。我们发现在整个剪接级联过程中，许多基因在增殖/减数分裂进入决定和生殖系性别决定中发挥作用，这表明剪接本身而不是剪接因子子集的新功能是这些过程所必需的。
In C. elegans, the decision between germline stem cell proliferation and entry into meiosis is controlled by GLP-1 Notch signaling, which promotes proliferation through repression of the redundant GLD-1 and GLD-2 pathways that direct meiotic entry. We identify prp-17 as another gene functioning downstream of GLP-1 signaling that promotes meiotic entry, largely by acting on the GLD-1 pathway, and which also functions in female germline sex determination. PRP-17 is orthologous to the yeast and human pre-mRNA splicing factor PRP17/CDC40 and can rescue the temperature-sensitive lethality of yeast PRP17. This link to splicing led to an RNAi screen of predicted C. elegans splicing factors in sensitized genetic backgrounds. We found that many genes throughout the splicing cascade function in the proliferation/meiotic entry decision and germline sex determination indicating that splicing per se, rather than a novel function of a subset of splicing factors, is necessary for these processes.
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