From the cellular perspective: exploring differences in the cellular baseline in macroscale and microfluidic cultures.
From the cellular perspective: exploring differences in the cellular baseline in macroscale and microfluidic cultures.
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DOI：
10.1039/b814565b
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发表时间：
2009-02
期刊：
影响因子：
--
通讯作者：
Beebe DJ
中科院分区：
文献类型：
--
作者：
Paguirigan AL;Beebe DJ
用于基于细胞培养的测定的微流体装置提供了新型的工程化微环境和用于控制和量化对这些微环境的细胞响应的新方法。然而，如果不从细胞的角度理解微器件中存在的微环境的影响，将微器件中完成的工作与通过传统方法获得的生物数据相结合将是具有挑战性的。随着细胞内蛋白质印迹（ICW）和原位分析技术对微流控装置的适应和验证，我们可以开始观察各种细胞对微培养物的反应。在这里，我们观察到在传统的宏观培养和微流体培养中培养的细胞之间的增殖，葡萄糖代谢，信号通路激活和蛋白质表达水平的几个差异。葡萄糖饥饿，生长因子限制，体积密度和与聚（二甲基硅氧烷）（PDMS）的相互作用的影响的问题进行了检查，以确定每个细胞行为的相对重要性。葡萄糖代谢的变化，对体积密度或培养基补充的不敏感性，以及最终随着暴露于PDMS的增加而减少的增殖，表明培养基/细胞与这种常用聚合物之间的相互作用可能对于一些基于细胞的测定是重要的。宏观和微流控培养中细胞之间的差异表明，由于规模的固有差异以及材料差异，细胞基线在微观培养中可能会发生实质性改变。这些观察结果突出了对用于基于细胞的测定的微流体装置进行生物学验证的需要，以便在传统的大量培养数据的背景下准确地解释用它们获得的数据。其他领域的研究，将进一步表征和验证微型文化进行了讨论。
Microfluidic devices for cell culture based assays provide new types of engineered microenvironments and new methods for controlling and quantifying cellular responses to these microenvironments. However, without an understanding of the effects of the microenvironments present in microdevices from a cellular perspective, it will be challenging to integrate work done in microdevices with biological data obtained via traditional methods. With the adaptation and validation of In Cell Westerns (ICWs) and in situ analysis techniques to microfluidic devices, we can begin to look at a variety of cellular responses to microcultures. Here we observe several differences in proliferation, glucose metabolism, signaling pathway activation and protein expression levels between cells cultured in traditional macroscale cultures and in microfluidic cultures. The issues of glucose starvation, growth factor restriction, volume density and effects of interactions with poly(dimethylsiloxane) (PDMS) were examined to determine the relative importance of each to cell behavior. Changes in glucose metabolism, insensitivity to volume density or media supplementation, and finally reduced proliferation as the exposure to PDMS increased, suggests that perhaps interactions between media/cells and this commonly employed polymer may be significant for some cell based assays. The differences between cells in macroscale and microfluidic cultures suggest that the cellular baseline may be substantially altered in microcultures due to both inherent differences in scale as well as material differences. The observations highlight the need to biologically validate micofluidic devices for cell based assays in order to accurately interpret the data obtained with them in the context of traditional macroculture data. Additional areas of study that will further characterize and validate microscale culture are discussed.
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影响因子：
7.4
作者：
Heo, Yun Seok;Cabrera, Lourdes M.;Takayama, Shuichi
通讯作者：
Takayama, Shuichi
影响因子：
4.8
作者：
Lee, AS
通讯作者：
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影响因子：
7.7
作者：
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通讯作者：
Patterson, C
影响因子：
6.1
作者：
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通讯作者：
Dufva, M.
影响因子：
6.1
作者：
Meyvantsson, Ivar;Warrick, Jay W.;Beebe, David J.
通讯作者：
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