Altered germline cyst formation and oogenesis in Tex14 mutant mice.
Altered germline cyst formation and oogenesis in Tex14 mutant mice.
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DOI：
10.1242/bio.058807
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发表时间：
2021-06-15
期刊：
影响因子：
2.4
通讯作者：
Lei L
中科院分区：
文献类型：
--
作者：
Ikami K;Nuzhat N;Abbott H;Pandoy R;Haky L;Spradling AC;Tanner H;Lei L
在小鼠胚胎卵巢卵母细胞分化过程中，姐妹生殖细胞通过细胞间桥连接，形成生殖细胞包囊。在包囊内，初级卵母细胞通过生殖细胞连接从姐妹生殖细胞获得细胞质和细胞器而形成。为了揭示细胞间桥在卵母细胞分化中的作用，我们分析了缺乏睾丸表达14（TEX14）的突变雌性小鼠，TEX14是一种参与细胞间桥形成和稳定的蛋白质。在Tex14纯合突变胎儿卵巢中，生殖细胞分裂形成数量减少的包囊，其中生殖细胞通过合胞体或破碎的细胞膜保持连接，而不是正常的细胞间桥。与野生型包囊相比，纯合子突变包囊以更高的频率破碎，产生的初级卵母细胞数量大大减少，具有早熟的胞质富集和体积增大。相比之下，Tex14杂合突变生殖系囊肿碎片较少，并产生初级卵母细胞在一个减少的大小。此外，纯合子突变体中增大的初级卵母细胞比过小的杂合子突变体初级卵母细胞更有效地用于维持卵泡发生。我们的观察结果直接将胚胎生殖系囊肿的性质与卵母细胞的分化和发育联系起来。总结：由于生殖细胞连接缺陷导致生殖系囊肿形成和破碎的改变导致Tex14突变小鼠卵母细胞分化和发育的变化。
During oocyte differentiation in mouse fetal ovaries, sister germ cells are connected by intercellular bridges, forming germline cysts. Within the cyst, primary oocytes form via gaining cytoplasm and organelles from sister germ cells through germ cell connectivity. To uncover the role of intercellular bridges in oocyte differentiation, we analyzed mutant female mice lacking testis-expressed 14 (TEX14), a protein involved in intercellular bridge formation and stabilization. In Tex14 homozygous mutant fetal ovaries, germ cells divide to form a reduced number of cysts in which germ cells remained connected via syncytia or fragmented cell membranes, rather than normal intercellular bridges. Compared with wild-type cysts, homozygous mutant cysts fragmented at a higher frequency and produced a greatly reduced number of primary oocytes with precocious cytoplasmic enrichment and enlarged volume. By contrast, Tex14 heterozygous mutant germline cysts were less fragmented and generate primary oocytes at a reduced size. Moreover, enlarged primary oocytes in homozygous mutants were used more efficiently to sustain folliculogenesis than undersized heterozygous mutant primary oocytes. Our observations directly link the nature of fetal germline cysts to oocyte differentiation and development. Summary: Altered germline cyst formation and fragmentation due to defective germ cell connectivity leads to changes in oocyte differentiation and development in Tex14 mutant mice.
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影响因子：
4.8
作者：
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