Variations in Oral Microbiota Composition Are Associated With a Risk of Throat Cancer
Variations in Oral Microbiota Composition Are Associated With a Risk of Throat Cancer
复制标题
口腔微生物群组成的变化与喉癌的风险相关
DOI：
10.3389/fcimb.2019.00205
复制
发表时间：
2019-07
期刊：
影响因子：
--
通讯作者：
Zhigang Huang
中科院分区：
文献类型：
--
作者：
Lili Wang;Gaofei Yin;Ying Guo;Yaqi Zhao;Meng Zhao;Yunyun Lai;Pengcheng Sui;Taiping Shi;Wei Guo;Zhigang Huang
In this study, a next-generation sequencing strategy on 16S ribosomal RNA (16S rRNA) gene was employed to analyze 70 oral samples from 32 patients with throat cancer, nine patients with vocal cord polyp, and 29 healthy individuals (normal controls). Using this strategy, we demonstrated, for the first time, that the salivary microbiota of cancer patients were significantly different from those of patients with a polyp and healthy individuals. We observed that the beta diversity of the cancer group was divergent from both the normal and polyp groups, while alpha-diversity indices such as the Chao1 estimator (P = 8.1e-05), Simpson (P = 0.0045), and Shannon (P = 0.0071) were significantly reduced in cancer patients compared with patients containing a polyp and normal healthy individuals. Linear discriminant analysis (LDA) and Kruskal–Wallis test analyses and real-time quantitative polymerase chain reaction (qPCR) verification test revealed that the genera Aggregatibacter, Pseudomonas, Bacteroides, and Ruminiclostridium were significantly enriched in the throat cancer group compared with the vocal cord polyp and normal control groups (score value >2). Finally, diagnostic models based on putatively important constituent bacteria were constructed with 87.5% accuracy [area under the curve (AUC) = 0.875, 95% confidence interval (CI): 0.695–1]. In summary, in this study we characterized, for the first time, the oral microbiota of throat cancer patients without smoking history. We speculate that these results will help in the pathogenic mechanism and early diagnosis of throat cancer.
登录
查看更多内容
影响因子：
3
作者：
Wendl MC
通讯作者：
Wendl MC
DOI：
10.1126/science.aan4526
发表时间：
2017-10-20
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Atarashi K;Suda W;Luo C;Kawaguchi T;Motoo I;Narushima S;Kiguchi Y;Yasuma K;Watanabe E;Tanoue T;Thaiss CA;Sato M;Toyooka K;Said HS;Yamagami H;Rice SA;Gevers D;Johnson RC;Segre JA;Chen K;Kolls JK;Elinav E;Morita H;Xavier RJ;Hattori M;Honda K
通讯作者：
Honda K
影响因子：
11.4
作者：
W. Zeng;G. Qiu;Hong-bo Zhou;J. Peng;Miao Chen;S. Tan;W. Chao;Xue-duan Liu;Yansheng Zhang
通讯作者：
W. Zeng;G. Qiu;Hong-bo Zhou;J. Peng;Miao Chen;S. Tan;W. Chao;Xue-duan Liu;Yansheng Zhang
影响因子：
3.7
作者：
Zeng XT;Deng AP;Li C;Xia LY;Niu YM;Leng WD
通讯作者：
Leng WD
印度喀拉拉邦Karunagappally的男性喉咙和咽癌风险的烟草使用和饮酒的关联 - 印度喀拉拉邦 - karunagappally队列研究。
影响因子：
3.7
作者：
Jayalekshmi PA;Nandakumar A;Akiba S;Gangadharan P;Koriyama C
通讯作者：
Koriyama C