Serotonin receptor B may lock the gate of PTTH release/synthesis in the Chinese silk moth, Antheraea pernyi; a diapause initiation/maintenance mechanism?
Serotonin receptor B may lock the gate of PTTH release/synthesis in the Chinese silk moth, Antheraea pernyi; a diapause initiation/maintenance mechanism?
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DOI：
10.1371/journal.pone.0079381
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Takeda M
中科院分区：
文献类型：
--
作者：
Wang Q;Mohamed AA;Takeda M
促胸激素（pth）的释放或阻断是中国蚕蛾（Antheraea pernyi）变态或蛹滞育发育通道的主要内分泌开关。我们克隆了编码两种类型5 -羟色胺受体（5HTRA和B）的cdna。5HTRA-和5htrb样免疫组织化学反应（-ir）与pth -ir共定位在原脑（DL）背外侧的两对神经分泌细胞中。因此，这些受体被怀疑与甲状旁腺激素的释放/合成有关。长日激活10个周期后，mRNA5HTRB水平下降到激活前的40%，而长日激活不影响5HTRA水平。在ld16:8和12:12条件下，注射dsRNA5HTRB使滞育终止提前，而注射dsRNA5HTRA对滞育终止率没有影响。注射dsRNA5HTRB诱导PTTH积累，表明5HTRB结合也抑制PTTH合成。这一结论在药理学上得到了支持；褪黑素受体拮抗剂luzindole加5在ld16∶8下抑制光周期激活，而5,7- dht在ld12∶12下诱导出窍，呈剂量依赖性。结果提示5HTRB可能锁定PTTH的释放/合成，维持滞育。这也可以作为滞育诱导机制。
The release of prothoracicotropic hormone, PTTH, or its blockade is the major endocrine switch regulating the developmental channel either to metamorphosis or to pupal diapause in the Chinese silk moth, Antheraea pernyi. We have cloned cDNAs encoding two types of serotonin receptors (5HTRA and B). 5HTRA-, and 5HTRB-like immunohistochemical reactivities (-ir) were colocalized with PTTH-ir in two pairs of neurosecretory cells at the dorsolateral region of the protocerebrum (DL). Therefore, the causal involvement of these receptors was suspected in PTTH release/synthesis. The level of mRNA5HTRB responded to 10 cycles of long-day activation, falling to 40% of the original level before activation, while that of 5HTRA was not affected by long-day activation. Under LD 16:8 and 12:12, the injection of dsRNA5HTRB resulted in early diapause termination, whereas that of dsRNA5HTRA did not affect the rate of diapause termination. The injection of dsRNA5HTRB induced PTTH accumulation, indicating that 5HTRB binding suppresses PTTH synthesis also. This conclusion was supported pharmacologically; the injection of luzindole, a melatonin receptor antagonist, plus 5th inhibited photoperiodic activation under LD 16:8, while that of 5,7-DHT, induced emergence in a dose dependent fashion under LD 12:12. The results suggest that 5HTRB may lock the PTTH release/synthesis, maintaining diapause. This could also work as diapause induction mechanism.
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影响因子：
10.6
作者：
Lucki, I
通讯作者：
Lucki, I
DOI：
10.1016/0742-8413(94)00101-f
发表时间：
1995-02-01
期刊：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY
影响因子：
--
作者：
SHIRAI, Y;SHIMAZAKI, K;AIZONO, Y
通讯作者：
AIZONO, Y
影响因子：
2.2
作者：
SHIRAI, Y;IWASAKI, T;AIZONO, Y
通讯作者：
AIZONO, Y
影响因子：
2.4
作者：
Rodriguez Moncalvo VG;Campos AR
通讯作者：
Campos AR
影响因子：
2.7
作者：
Sauman, I;Reppert, SM
通讯作者：
Reppert, SM