Effects of thiazolidinediones on growth and differentiation of human aorta and coronary myocytes
Effects of thiazolidinediones on growth and differentiation of human aorta and coronary myocytes
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噻唑烷二酮类药物对人主动脉和冠状肌细胞生长和分化的影响
DOI：
10.1016/s0895-7061(97)90528-8
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发表时间：
1997
影响因子：
3.2
通讯作者：
H. Pershadsingh
中科院分区：
文献类型：
--
作者：
E. Morikang;S. Benson;T. Kurtz;H. Pershadsingh
噻唑烷二酮是胰岛素增敏剂，在胰岛素抵抗状态下降低胰岛素和脂质水平，在体外阻断激动剂诱导的Ca2+进入血管平滑肌（VSM）细胞，并在体内降低动物和人类的血压。在本研究中，我们研究了西格列酮和曲格列酮对培养的人主动脉（haVSM）和人冠状动脉（hcaVSM） VSM细胞生长、DNA合成（胸苷结合）和分化的影响。用血清或血小板衍生生长因子（PDGF）刺激有丝分裂静止的haVSM细胞。西格列酮（40μmol/L）对haVSM细胞增殖的抑制作用为84±16%（平均±SEM） （P< 0.05, n = 3），血清和PDGF对[3H]-胸腺嘧啶结合的抑制作用分别为91±18% （P< 0.01, n = 3）和73±14% （P< 0.01, n = 4）。曲格列酮（5μmol/L）对haVSM细胞增殖的抑制作用为78±14% (P< 0.05, n = 3)，对hcaVSM细胞的抑制作用为91±18% （P< 0.05, n = 3）。增殖型VSM细胞（合成表型）表达少量α-肌动蛋白，而非增殖型VSM细胞（收缩表型）表达丰富的α-肌动蛋白。在40μmol/L西格列酮的作用下，增殖的haVSM细胞α-肌动蛋白染色明显增加，与向分化良好的收缩表型过渡一致。在某种程度上，噻唑烷二酮类药物类似地影响生长因子诱导的动脉肌细胞的增殖和分化，这些药物可能有助于治疗动脉粥样硬化和预防血管成形术后再狭窄。©1997美国高血压杂志有限公司Am J Hypertens 1997；10:428 - 433
Thiazolidinediones, insulin-sensitizing agents that lower insulin and lipid levels in insulin-resistant states, block agonist-induced Ca2+entry into vascular smooth muscle (VSM) cells in vitro and lower blood pressure in animals and humans in vivo. In this study, we investigated the effects of ciglitazone and troglitazone on cell growth and DNA synthesis (as thymidine incorporation), and differentiation in cultured human aorta (haVSM) and human coronary artery (hcaVSM) VSM cells. Mitotically quiescent haVSM cells were stimulated with serum or platelet-derived growth factor (PDGF). Ciglitazone (40μmol/L) inhibited haVSM cell proliferation by 84 ± 16% (mean ± SEM) (P< .05, n = 3), and serum and PDGF stimulated [3H]-thymidine incorporation by 91 ± 18% (P< .03, n = 3) and 73 ± 14% (P< .03, n = 4), respectively. Troglitazone (5μmol/L) inhibited proliferation of haVSM cells by 78 ± 14% (P< .05, n = 3) and hcaVSM cells by 91 ± 18% (P< .05, n = 3). Proliferating VSM cells (synthetic phenotype) expressed small amounts ofα-actin, whereas nonproliferating VSM cells (contractile phenotype) exhibited abundantα-actin. Exposure of proliferating haVSM cells to 40μmol/L ciglitazone induced a marked increase inα-actin staining, consistent with transition to the well differentiated, contractile phenotype. To the extent that thiazolidinediones similarly affect growth factor-induced proliferation and differentiation of arterial myocytes in vivo, these agents may be useful in treating atherosclerosis and in preventing restenosis after angioplasty. © 1997 American Journal of Hypertension, Ltd. Am J Hypertens 1997;10:428–433
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影响因子：
2.2
作者：
KORZENIEWSKI, C;CALLEWAERT, DM
通讯作者：
CALLEWAERT, DM
影响因子：
15.9
作者：
JAWIEN, A;BOWENPOPE, DF;CLOWES, AW
通讯作者：
CLOWES, AW
影响因子：
56.9
作者：
ROSS, R;MASUDA, J;SATO, H
通讯作者：
SATO, H
吡格列酮对血管平滑肌钙通道的影响。
DOI：
10.1161/01.hyp.24.2.170
发表时间：
1994
期刊：
Hypertension (Dallas, Tex. : 1979)
影响因子：
--
作者：
Zhang,F;Sowers,JR;Ram,JL;Standley,PR;Peuler,JD
通讯作者：
Peuler,JD
影响因子：
158.5
作者：
NOLAN, JJ;LUDVIK, B;OLEFSKY, J
通讯作者：
OLEFSKY, J