Expression of the c-kit receptor in human lymphomas is restricted to Hodgkin's disease and CD30+ anaplastic large cell lymphomas.
Expression of the c-kit receptor in human lymphomas is restricted to Hodgkin's disease and CD30+ anaplastic large cell lymphomas.
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c-kit受体在人类淋巴瘤中的表达仅限于霍奇金病和CD30间变性大细胞淋巴瘤。
作者：
Antonio Pinto;A. Gloghini;Valter Gattei;D. Aldinucci;V. Zagonel;A. Carbone
原癌基因c-kit的产物是一种跨膜受体蛋白，其通过与其称为干细胞因子的特异性配体相互作用在正常和肿瘤性造血的调节中起重要作用。为了检查c-kit产物是否可能参与人类淋巴瘤的发病机制，我们通过用17 F11抗体对淋巴结冷冻切片进行免疫染色，检测c-kit受体的细胞外表位，并通过北方印迹杂交检测c-kit RNA，分析了c-kit蛋白在来自各种淋巴肿瘤的肿瘤细胞中的表达。在24例B和T细胞表型的非霍奇金淋巴瘤（NHL）中，无一表达免疫可检测的c-kit蛋白，该蛋白在反应性淋巴结和正常扁桃体的淋巴样细胞中也未得到证实。与此相反，c-kit蛋白表达的Reed-Sternberg细胞和他们的单核细胞变体从11 21霍奇金病（HD）的情况下，和11 16例CD 30+间变性大细胞淋巴瘤（ALCL）的肿瘤细胞。c-kit特异性mRNA在HD和ALCL患者的淋巴结组织中也有表达，但在NHL的肿瘤组织中没有表达。此外，通过流式细胞术证实了一例ALCL大量累及骨髓的患者中的c-kit/CD 30+肿瘤细胞。排除HD和ALCL中导致c-kit表达的淋巴细胞优势HD病例和HD的其他组织学亚型或肿瘤细胞的免疫表型（B、T、非B-非T）。c-kit产物在HD和ALCL的人类淋巴瘤中的高度限制性表达为这两种密切相关的淋巴瘤实体之间提供了进一步的生物学联系。
The product of the proto-oncogene c-kit is a transmembrane receptor protein that plays an important role in the regulation of normal and neoplastic hematopoiesis via the interaction with its specific ligand termed stem cell factor. To examine whether c-kit product is possibly involved in the pathogenesis of human lymphomas, we analyzed the expression of the c-kit protein in neoplastic cells from a variety of lymphoid tumors by immunostaining of lymph node frozen sections with the 17F11 antibody, detecting an extracellular epitope of the c-kit receptor, and of c-kit RNA by Northern blot hybridization. Of 24 nonHodgkin's lymphomas (NHL) of B- and T-cell phenotype, none expressed immunodetectable c-kit protein that was also not evidenced in lymphoid cells of reactive lymph nodes and normal tonsils. In contrast, c-kit protein was expressed by Reed-Sternberg cells and their mononuclear variants from 11 of 21 Hodgkin's disease (HD) cases, and in tumor cells from 11 of 16 cases of CD30+ anaplastic large cell lymphoma (ALCL). c-kit specific mRNA was also detected in lymph node tissues from HD and ALCL cases but not in neoplastic tissues from NHL other than ALCL. In addition, c-kit/CD30+ tumor cells were evidenced by flow cytometry in a patient displaying massive bone marrow involvement by ALCL. With the exclusion of lymphocyte predominance cases of HD that resulted c-kit expression and the other histologic subtypes of HD or the immunologic phenotype of tumor cells (B, T, nonB-nonT) in both HD and ALCL. The highly restricted expression of the c-kit product among human lymphomas to HD and ALCL provides a further biologic link between these two closely related lymphoma entities.
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影响因子：
20.3
作者：
Anne M. Turner;K. Zsebo;FH Martin;Fredrick W. Jacobsen;Larry G. Bennett;V. C. Broudy
通讯作者：
Anne M. Turner;K. Zsebo;FH Martin;Fredrick W. Jacobsen;Larry G. Bennett;V. C. Broudy
影响因子：
10.5
作者：
NOCKA, K;MAJUMDER, S;BESMER, P
通讯作者：
BESMER, P
影响因子：
2.9
作者：
Sumbele S;Fotelli MN;Nikolopoulos D;Tooulakou G;Liakoura V;Liakopoulos G;Bresta P;Dotsika E;Adams MA;Karabourniotis G
通讯作者：
Karabourniotis G
DOI：
--
发表时间：
1991-12
期刊：
Cancer cells
影响因子：
--
作者：
H. Broxmeyer;R. Maze;Keisuke Miyazawa;C. Carow;Paul C. Hendrie;S. Cooper;G. Hangoc;Saroj Vadhan-Raj;Li Lu
通讯作者：
H. Broxmeyer;R. Maze;Keisuke Miyazawa;C. Carow;Paul C. Hendrie;S. Cooper;G. Hangoc;Saroj Vadhan-Raj;Li Lu
影响因子：
20.3
作者：
E. Vannier;Sally Cowley;Shuxian Jiang;S. Chi;C. Dinarello;K. Zsebo
通讯作者：
E. Vannier;Sally Cowley;Shuxian Jiang;S. Chi;C. Dinarello;K. Zsebo