The tarsal taste of honey bees: behavioral and electrophysiological analyses.
The tarsal taste of honey bees: behavioral and electrophysiological analyses.
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蜜蜂的跗骨味道：行为和电生理分析
DOI：
10.3389/fnbeh.2014.00025
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发表时间：
2014
影响因子：
3
通讯作者：
Giurfa M
中科院分区：
文献类型：
--
作者：
de Brito Sanchez MG;Lorenzo E;Su S;Liu F;Zhan Y;Giurfa M
味道在蜜蜂的生活中起着至关重要的作用，因为它们的生存依赖于花蜜和花粉以及其他天然产品的收集和摄取。在这里，我们通过一系列的行为和电生理分析来研究蜜蜂的鞑靼味道。我们表征了对传递给前鞑靼味觉感受器的各种甜味、咸味和苦味的反应。行为学实验表明，用蔗糖、苦味物质或水刺激对侧前鞑靼，根据刺激顺序的不同，产生不同的结果。当首先施加蔗糖，从而引起鼻子伸展时，没有苦味物质能引起鼻子回缩，这表明蔗糖的优先刺激引起了中枢兴奋状态。当首先施加苦味物质或水时，蔗糖刺激仍可引起鼻孔伸展，但水平较低，这表明中枢抑制是基于相反的味觉输入对对面鞑靼的影响。电生理实验表明，在低浓度的生理盐水溶液中，位于眼球味觉感受器中的感受器细胞对此高度敏感。在这些感受器中没有发现受体对蔗糖或苦味物质有特殊反应的证据。爪子味觉感受器中的受体细胞对蔗糖高度敏感。虽然蜜蜂在眼镜蛇中没有专门的苦味受体，但间接检测苦味是可能的，因为苦味在与蔗糖溶液混合时抑制了爪子的蔗糖受体细胞。通过结合行为学和电生理学的方法，这些结果提供了第一个在蜜蜂中进行鞑靼味道检测的综合性研究。
Taste plays a crucial role in the life of honey bees as their survival depends on the collection and intake of nectar and pollen, and other natural products. Here we studied the tarsal taste of honey bees through a series of behavioral and electrophysiological analyses. We characterized responsiveness to various sweet, salty and bitter tastants delivered to gustatory sensilla of the fore tarsi. Behavioral experiments showed that stimulation of opposite fore tarsi with sucrose and bitter substances or water yielded different outcomes depending on the stimulation sequence. When sucrose was applied first, thereby eliciting proboscis extension, no bitter substance could induce proboscis retraction, thus suggesting that the primacy of sucrose stimulation induced a central excitatory state. When bitter substances or water were applied first, sucrose stimulation could still elicit proboscis extension but to a lower level, thus suggesting central inhibition based on contradictory gustatory input on opposite tarsi. Electrophysiological experiments showed that receptor cells in the gustatory sensilla of the tarsomeres are highly sensitive to saline solutions at low concentrations. No evidence for receptors responding specifically to sucrose or to bitter substances was found in these sensilla. Receptor cells in the gustatory sensilla of the claws are highly sensitive to sucrose. Although bees do not possess dedicated bitter-taste receptors in the tarsi, indirect bitter detection is possible because bitter tastes inhibit sucrose receptor cells of the claws when mixed with sucrose solution. By combining behavioral and electrophysiological approaches, these results provide the first integrative study on tarsal taste detection in the honey bee.
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影响因子：
15.9
作者：
Carleton A;Accolla R;Simon SA
通讯作者：
Simon SA
DOI：
10.1002/neu.20063
发表时间：
2004-12-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
Hiroi, M;Meunier, N;Tanimura, T
通讯作者：
Tanimura, T
DOI：
10.1007/s003590100191
发表时间：
2001-04-01
影响因子：
2.1
作者：
Bernays, EA;Chapman, RF
通讯作者：
Chapman, RF
影响因子：
2.2
作者：
Bernays, EA;Chapman, RF
通讯作者：
Chapman, RF
影响因子：
3.7
作者：
Ayestaran A;Giurfa M;de Brito Sanchez MG
通讯作者：
de Brito Sanchez MG