Genome-Wide Identification of the ERF Transcription Factor Family for Structure Analysis, Expression Pattern, and Response to Drought Stress in Populus alba × Populus glandulosa.
Genome-Wide Identification of the ERF Transcription Factor Family for Structure Analysis, Expression Pattern, and Response to Drought Stress in Populus alba × Populus glandulosa.
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DOI：
10.3390/ijms24043697
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发表时间：
2023-02-12
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
乙烯反应因子（ERF）转录因子家族对于调节植物生长和逆境反应非常重要。虽然ERF家族成员在许多植物中的表达模式已被报道，但其在重要的森林研究模式植物银白杨中的作用仍不清楚。本研究通过对银白杨×腺白杨基因组的分析，鉴定了209个PagERF转录因子。分析了它们的氨基酸序列、分子量、理论pI（等电点）、不稳定指数、脂肪族指数、总平均亲水性和亚细胞定位。大多数PagERF被预测定位在细胞核中，只有少数PagERF定位在细胞质和细胞核中。系统发育分析将PagERF蛋白分为10组，I类至X类，属于同一组的蛋白含有相似的基序。分析了PagERF基因启动子中与植物激素、非生物胁迫反应和MYB结合位点相关的顺式作用元件。利用转录组数据分析了PagERF基因在银白杨腋芽、幼叶、功能叶、形成层、木质部和根等不同组织中的表达模式，结果表明PagERF基因在银白杨各组织中均有表达，尤其是根。定量验证结果与转录组数据一致。用6%聚乙二醇6000（PEG 6000）处理银白杨幼苗，RT-qRCR结果表明，9个PagERF基因在不同组织中对干旱胁迫有响应。该研究为PagERF家族成员在调节植物生长发育和胁迫响应中的作用提供了新的视角。本研究为ERF家族的进一步研究提供了理论基础。
The Ethylene Responsive Factor (ERF) transcription factor family is important for regulating plant growth and stress responses. Although the expression patterns of ERF family members have been reported in many plant species, their role in Populus alba × Populus glandulosa, an important model plant for forest research, remains unclear. In this study, we identified 209 PagERF transcription factors by analyzing the P. alba × P. glandulosa genome. We analyzed their amino acid sequences, molecular weight, theoretical pI (Isoelectric point), instability index, aliphatic index, grand average of hydropathicity, and subcellular localization. Most PagERFs were predicted to localize in the nucleus, with only a few PagERFs localized in the cytoplasm and nucleus. Phylogenetic analysis divided the PagERF proteins into ten groups, Class I to X, with those belonging to the same group containing similar motifs. Cis-acting elements associated with plant hormones, abiotic stress responses, and MYB binding sites were analyzed in the promoters of PagERF genes. We used transcriptome data to analyze the expression patterns of PagERF genes in different tissues of P. alba × P. glandulosa, including axillary buds, young leaves, functional leaves, cambium, xylem, and roots, and the results indicated that PagERF genes are expressed in all tissues of P. alba × P. glandulosa, especially in roots. Quantitative verification results were consistent with transcriptome data. When P. alba × P. glandulosa seedlings were treated with 6% polyethylene glycol 6000 (PEG6000), the results of RT-qRCR showed that nine PagERF genes responded to drought stress in various tissues. This study provides a new perspective on the roles of PagERF family members in regulating plant growth and development, and responses to stress in P. alba × P. glandulosa. Our study provides a theoretical basis for ERF family research in the future.
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