Bifunctional nano-Ag(3)PO(4) with capabilities of enhancing ceftazidime for sterilization and removing residues.
Bifunctional nano-Ag(3)PO(4) with capabilities of enhancing ceftazidime for sterilization and removing residues.
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双功能纳米Ag3PO4增强头孢他啶杀菌及去除残留能力
作者：
由于抗生素对细菌的效力随着时间的推移而降低，抗生素排放量的增加已成为一个日益严重的问题。本研究提出了一种无污染残留的纳米抗菌技术，即利用无机纳米Ag3PO4协同增强头孢他啶的抗菌活性，然后通过纳米银去除药物残留。采用简单的离子交换法合成了Ag_3PO_4，不使用任何还原剂和保护剂。首次研究了Ag3PO4与22种抗生素对大肠杆菌的联合抗菌作用。结果表明，Ag3PO4与头孢他啶的协同作用最好。其次，提出了协同作用的机理，并通过zeta电位分析仪、X射线光电子能谱（XPS）和红外光谱（IR）测定了Ag3PO4与头孢他啶之间的非化学键力。通过表面等离子体共振光谱（SPR）、扫描电镜（SEM）和碘化丙啶（PI）染色进一步证明抗菌剂与细菌之间的相互作用。此外，研究了活性氧（ROS）的产生、氧化应激的诱导以及银离子在Ag3PO4中的溶解，发现只有在光照下，才能产生ROS。结论：Ag3PO4和头孢他啶协同作用是导致细胞壁破坏的主要原因。
Since the efficacy of antibiotics towards bacteria is decreasing over time, the rising of antibiotic emission has become an increasingly grave issue. In this study, we proposed an integrated antibacterial nanotechnology without pollution residues, which synergistically enhances the antibacterial activity of ceftazidime by using the inorganic nano-Ag3PO4, and subsequently removes drug residues by photocatalysis. Ag3PO4 were synthesized using a simple ion-exchange method without any reducing agent or protectant. The combined antibacterial activity of Ag3PO4 and 22 kinds of antibiotics against Escherichia coli was first studied. The results showed that Ag3PO4 and ceftazidime exhibited the best synergistic effect. Next, the synergy mechanism was proposed, the non-chemical bond forces between Ag3PO4 and ceftazidime was determined by zeta potential analyzer, X-ray photoelectron spectroscopy (XPS) and infrared spectroscopy (IR). The interaction between antimicrobials and bacteria was further demonstrated by surface plasma resonance spectroscopy (SPR), scanning electron microscopy (SEM) and propidium iodide (PI) staining. In addition, the production of reactive oxygen species (ROS), the induction of oxidative stress and dissolution of silver ions in Ag3PO4 were studied and found out that only under light, could the ROS be generated. In conclusion, the synergistic effect of Ag3PO4 and ceftazidime is responsible for the joint destruction of cell wall.
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